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Species: firefly
Genetic Insert: FKBP-Luciferase fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2280; Vector Backbone:pENTR1A; Vector Types:Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17455 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2427; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 3x HA tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334817
Proper citation: RRID:Addgene_17456 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2595; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 6x MYC tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334818
Proper citation: RRID:Addgene_17457 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3030; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal ECFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU344820
Proper citation: RRID:Addgene_17459 Copy
Species: Caenorhabditis elegans
Genetic Insert: htz-1 targeting gRNA
Vector Backbone Description: Vector Backbone:pTK73; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34865044
Proper citation: RRID:Addgene_174546 Copy
Species: Other
Genetic Insert: Venus with golgi apparatus-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174472 Copy
Species: Other
Genetic Insert: Sirius with actin-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174475 Copy
Species: Other
Genetic Insert: EGFP with actin-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174477 Copy
Vector Backbone Description: Vector Backbone:pOPIN-A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_174581 Copy
Species: Homo sapiens
Genetic Insert: VPS29
Vector Backbone Description: Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18981234
Proper citation: RRID:Addgene_174520 Copy
Species: Mus musculus
Genetic Insert: MED20
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15435 Copy
Species: Homo sapiens
Genetic Insert: MED22
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5900; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15432 Copy
Species: Mus musculus
Genetic Insert: MED19
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5900; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15430 Copy
Species: Caenorhabditis elegans
Genetic Insert: linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154316 Copy
Species: Caenorhabditis elegans
Genetic Insert: linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154317 Copy
Species: Caenorhabditis elegans
Genetic Insert: linker::3xMyc for CT slot
Vector Backbone Description: Backbone Size:2361; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154318 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::AID*::3xFLAG for NT slot
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154314 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::TEV::AID*::3xFLAG for NT slot
Vector Backbone Description: Backbone Size:478; Vector Backbone:Topo-Blunt; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154315 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa Linker for CT slot
Vector Backbone Description: Backbone Size:478; Vector Backbone:Topo-Blunt; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154310 Copy
Species: Homo sapiens
Genetic Insert: O-GlcNAc Transferase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pET24b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16231908
Proper citation: RRID:Addgene_154271 Copy
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