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Species: Other
Genetic Insert: mRuby3
Vector Backbone Description: Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34100715
Proper citation: RRID:Addgene_172854 Copy
Species: Synthetic
Genetic Insert: (GGS)2G
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137097 Copy
Species: Synthetic
Genetic Insert: (GGS)4GSG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137099 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized GFP
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137021 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized Halo Tag
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137023 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66635.2
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137046 Copy
Genetic Insert: blasticidin-S deaminase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137049 Copy
Species: Other
Genetic Insert: puromycin N-acetyl-transferase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137050 Copy
Vector Backbone Description: Vector Backbone:GOI flox pDONR221; Vector Types:Leishmania floxed gene expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137055 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAF07416.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. RevA is a lipoprotein. RevA is not expected to be lipidated in this construct. Following TEV cleavage, RevA lacks 5 residues (CKAYV) at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137064 Copy
Species: Synthetic
Genetic Insert: G(TP)4TG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137101 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66700.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. BB0323 is a lipoprotein. BB0323 is not expected to be lipidated in this construct. After TEV cleavage, the target protein has an added A residue at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137067 Copy
Species: Synthetic
Genetic Insert: GGASPAGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137102 Copy
Species: Synthetic
Genetic Insert: GGA(EAAAK)2AGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137104 Copy
Species: Synthetic
Genetic Insert: FKBP12
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137108 Copy
Species: Synthetic
Genetic Insert: AI(TVMV)
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137111 Copy
Species: Synthetic
Genetic Insert: GG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137091 Copy
Species: Synthetic
Genetic Insert: GPG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137093 Copy
Species: Synthetic
Genetic Insert: GGSG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137095 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
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