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Species: Homo sapiens
Genetic Insert: 3x MHC I KB element - luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7707523
Comments: The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).
Proper citation: RRID:Addgene_26699 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20551166
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20729842
Proper citation: RRID:Addgene_26768 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26775 Copy
Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8688416
Comments: There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
Proper citation: RRID:Addgene_26774 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-LIN28A-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26819 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-OCT4-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that OCT4 has some silent mutations.
Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26816 Copy
Species: Homo sapiens
Genetic Insert: dystrophin
Vector Backbone Description: Backbone Size:6656; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral, lentiviral plasmid backbone from L. Naldini; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: Human (alpha) Skeletal Actin (HSA) promotor
Vp intron
Proper citation: RRID:Addgene_26810 Copy
Species: Homo sapiens
Genetic Insert: NICD3
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508304
Comments: EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos
A1757T mutation in Notch3 is not known to affect function.
Proper citation: RRID:Addgene_26894 Copy
Species: Homo sapiens
Genetic Insert: Neurofibromatosis type I
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:5524; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8628317
Comments: The authors note that there is a mutation in the coding sequence: C3918T
"This created a T to I change within the GAP domain of NF1. However, this
change is outside of the conserved blocks within the GAP domain. This
particular residue (1195 of NF1-GRD shown in figure 4 in the chapter by
M.R. Kim and F. Tamanoi, chapter 6 in Neurofibromatosis type I from
genotype to phenotype, editor by M. Upadhyaya and D. N. Cooper BIOS
scientific publisher) is not conserved among different GAP proteins.
Therefore, we believe that the amino acid change does not affect the GAP
activity. In fact, we have used this construct for the wild-type GAP
activity."
Proper citation: RRID:Addgene_19993 Copy
Species: Homo sapiens
Genetic Insert: Insulin-like growth factor 2 mRNA binding protein 3
Vector Backbone Description: Backbone Size:5862; Vector Backbone:pDESTmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19879 Copy
Species: Homo sapiens
Genetic Insert: F-box and WD repeat domain containing 5 delta F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18381890
Comments: F-Box Deletion Mutant
(L45M mutation present but does not affect function)
Proper citation: RRID:Addgene_19912 Copy
Species: Homo sapiens
Genetic Insert: Eukaryotic translation initiation factor 2C, 4
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19890 Copy
Species: Homo sapiens
Genetic Insert: Poly(A) binding protein, cytoplasmic 4
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19882 Copy
Species: Homo sapiens
Genetic Insert: Eukaryotic translation initiation factor 2C, 3
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19889 Copy
Species: Homo sapiens
Genetic Insert: PCTAIRE-1
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1998 Copy
Species: Homo sapiens
Genetic Insert: GfaABC1D-tTA
Vector Backbone Description: Backbone Size:7408; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19977 Copy
Species: Homo sapiens
Genetic Insert: Fyn
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8206991
Proper citation: RRID:Addgene_19979 Copy
Species: Homo sapiens
Genetic Insert: SGK1 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19865 Copy
Species: Homo sapiens
Genetic Insert: RBJ 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19866 Copy
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