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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
PKC zeta DN
 
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1+ mentions
RRID:Addgene_21249 PKC zeta Rattus norvegicus Ampicillin PMID:12794082 Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K281M 2026-08-29 01:02:07 2
PKC epsilon DN
 
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RRID:Addgene_21243 PKC epsilon Mus musculus Ampicillin PMID:12794082 Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K437R 2026-08-29 01:02:06 2
PKC zeta WT
 
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RRID:Addgene_21248 PKC zeta Rattus norvegicus Ampicillin PMID:12794082 Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:03 1
PKC eta CAT
 
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RRID:Addgene_21247 PKC eta Mus musculus Ampicillin PMID:12794082 Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440. Please note that Addgene was not able to completely confirm the truncation with quality control sequencing. Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin delta 1-341 2026-08-29 01:02:01 1
PKC epsilon WT
 
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RRID:Addgene_21240 PKC epsilon Mus musculus Ampicillin PMID:12794082 Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:06 2
GFP-C1(2)delta
 
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1+ mentions
RRID:Addgene_21216 GFP-N2-PKCdelta-C1 Rattus norvegicus Kanamycin PMID:11509231 Backbone Marker:Clontech; Backbone Size:4596; Vector Backbone:N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin C1(2) domains of PKC delta (rat) (Lab plasmid WC0001) 2026-08-29 01:02:00 6
PGK-H2BeGFP
 
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10+ mentions
RRID:Addgene_21210 Phosphoglycerate kinase promoter Homo sapiens Ampicillin PMID:19352491 There are several mismatches between depositor's and Addgene sequence. These mutations do not affect eGFP expression. Backbone Size:7226; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:00 15
GFP-C2gamma
 
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RRID:Addgene_21215 GFP-pcDNA3-PKCgamma-c2 Rattus norvegicus Ampicillin PMID:11509231 Backbone Size:5429; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin The C2-domain from PKCgamma (aa170-260) plus flanking sequence. This insert is thus amino acids 158-284 from PKCgamma (rat). (Lab plasmid WC 0005) 2026-08-29 01:02:06 3
MSCV JMJD3 mutant
 
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RRID:Addgene_21214 JMJD3 Homo sapiens Ampicillin PMID:18628393 Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin H1390A 2026-08-29 01:02:03 7
PM-GFP
 
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10+ mentions
RRID:Addgene_21213 PM-GFP Ampicillin PMID:10598863 Backbone Size:5419; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin PM targetting sequence is Lyn (MGCIKSKRKD) (Lab plasmid WC 0017) 2026-08-29 01:02:00 10
GFP-C1-PLCdelta-PH
 
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50+ mentions
RRID:Addgene_21179 PLCdelta Homo sapiens Kanamycin PMID:9512420 This construct encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids (Stauffer TP, Ahn S, Meyer T; Curr Biol. 1998 8:343-6). It is best used in combination with a second fluorescent protein that uniformly marks the plasma membrane (the last 20 amino acids "CAAX" region of KRas is often used as a PM marker). Relative changes in cell-wide or local plasma membrane PI(4,5)P2 can be determined by using a mask for the PM region and by dividing the two background subtracted PH domain and CAAX images. At high expression levels, this construct interferes with PI(4,5)P2 signaling and alters cortical actin (Raucher D et al Cell. 2000 100:221-8). Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin PH domain of human PLC delta, amino acids Met1–Ile175. (Lab plasmid WC 0003) 2026-08-29 01:02:02 96
MSCV JMJD3
 
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1+ mentions
RRID:Addgene_21212 JMJD3 Homo sapiens Ampicillin PMID:18628393 Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:06 9
aMHC-eGFP-Rex-Neo
 
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1+ mentions
RRID:Addgene_21229 alpha myosin heavy chain promoter Mus musculus Ampicillin PMID:19352491 Backbone Size:8400; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:00 9
aMHC-mCherry-Rex-Blasticidin
 
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1+ mentions
RRID:Addgene_21228 alpha myosin heavy chain promoter Mus musculus Ampicillin PMID:19352491 There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct. Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:06 5
pRosa26-DEST
 
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10+ mentions
RRID:Addgene_21189 GateWay conversion cassette Chloramphenicol and Ampicillin PMID:19014667 pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct. Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-29 01:02:06 18
GFP-C1-CAMKIIalpha-T286A
 
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RRID:Addgene_21220 CAMKIIalpha-T286A Rattus norvegicus Kanamycin PMID:10102820 Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T286A (Lab plasmid KS 0003). 2026-08-29 01:02:00 1
GFP-C1-CAMKIIalpha
 
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10+ mentions
RRID:Addgene_21226 CAMKIIalpha Rattus norvegicus Kanamycin PMID:9768845 Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Lab plasmid KS 0001. 2026-08-29 01:02:00 10
PL-SIN-EOS-S(4+)-EGFP
 
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1+ mentions
RRID:Addgene_21317 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 1
pMXs-mRFP1
 
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1+ mentions
RRID:Addgene_21315 monomeric Red Fluorescence Protein 1 Ampicillin PMID:19404254 mRFP1 (maximum Excitation wave length, 584 nm; maximum Emission wave length, 607 nm). pMXs MoMLV-based retroviral vector from Dr. Toshio Kitamura (Institute of Medical Science, University of Tokyo). Backbone Size:4582; Vector Backbone:pMXs MoMLV-based retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:01 5
Axin2 probe
 
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RRID:Addgene_21277 Axin2 Mus musculus Ampicillin PMID:11809808 Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:For riboprobe synthesis; Bacterial Resistance:Ampicillin cDNA bp 1774 to 2787 2026-08-29 01:02:03 4

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