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Species: Orthoreovirus T1L
Genetic Insert: L1
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20042210
Proper citation: RRID:Addgene_33303 Copy
Species: Orthoreovirus T3D
Genetic Insert: S2
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20042210
Proper citation: RRID:Addgene_33302 Copy
Species: uncultured proteobacterium EBAC31A08
Genetic Insert: Proteorhodopsin Optical Proton Sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4127; Vector Backbone:pBAD TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21764748
Proper citation: RRID:Addgene_33780 Copy
Species: firefly
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:David Baltimore; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21439256
Proper citation: RRID:Addgene_33307 Copy
Genetic Insert: HSV-TK/GFP
Vector Backbone Description: Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19114988
Proper citation: RRID:Addgene_33308 Copy
Species: Mus musculus
Genetic Insert: Etv3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18025162
Proper citation: RRID:Addgene_33374 Copy
Species: Mus musculus
Genetic Insert: Etv3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18025162
Proper citation: RRID:Addgene_33375 Copy
Species: E. coli
Genetic Insert: Improved MBP based Maltose Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21989929
Comments: The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5.
Proper citation: RRID:Addgene_33373 Copy
Species: Homo sapiens
Genetic Insert: TCPTP-45
Vector Backbone Description: Backbone Marker:Vanberbilt structural biology core plasmid; Vector Backbone:pBG100; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20940300
Proper citation: RRID:Addgene_33365 Copy
Species: Homo sapiens
Genetic Insert: Cav1
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20940300
Proper citation: RRID:Addgene_33364 Copy
Genetic Insert: TetR cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pCRII; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones the Tet cassette has been extremely reliable.
Proper citation: RRID:Addgene_33359 Copy
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pGEM; Vector Types:Trypanosoma brucei expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18006158
Comments: This plasmid is also known as pLEW100creTS-SAS.
Cre recombinase is flanked by UTR sequences to allow regulated T7 promoter-driven low level expression after cleavage by NotI and integration into a rRNA locus.
Proper citation: RRID:Addgene_33344 Copy
Genetic Insert: CreERT2
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20595231
Proper citation: RRID:Addgene_33341 Copy
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pGEM; Vector Types:Cre/Lox, Trypanosoma brucei expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18006158
Comments: This plasmid is also known as pLEW100creTS.
Cre recombinase is flanked by UTR sequences to allow regulated T7 promoter-driven low level expression after cleavage by NotI and integration into a rRNA locus.
Proper citation: RRID:Addgene_33345 Copy
Species: Firefly
Genetic Insert: Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
Vector Backbone Description: Backbone Size:4417; Vector Backbone:pGEM; Vector Types:Trypanosoma brucei expression vector; Bacterial Resistance:Ampicillin
Comments: This plasmid is a derivative of Addgene plasmid 24011: pLEW100v5.
Proper citation: RRID:Addgene_33346 Copy
Vector Backbone Description: Backbone Marker:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14702347
Comments: The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with NdeI and HindIII, and the following annealed oligonucleotide was inserted with a hemagglutinin epitope (YPYDVPDYASL) and NdeI-HindIII cloning site was made using the following pair of complementary oligonucleotides: 5′-AGCTCCACCATGGCGTATCCCTACGACGTGCCCGATTATGCCCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGGGCATAATCGGGCACGTCGTAGGGATACGCCATGGTGG-3′ (underlined bases are restriction site for XbaI).
Proper citation: RRID:Addgene_33349 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33332 Copy
Species: Mus musculus
Genetic Insert: Atoh1
Vector Backbone Description: Backbone Marker:Addgene plasmid #33336; Backbone Size:6575; Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20516124
Proper citation: RRID:Addgene_33333 Copy
Vector Backbone Description: Backbone Size:3744; Vector Backbone:pBPXcm-1; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_33298 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase alpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33331 Copy
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