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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Axin2-luciferase
 
Resource Report
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1+ mentions
RRID:Addgene_21275 Axin2 promoter Mus musculus Ampicillin PMID:11809808 A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF binding sites (underlined and designated T1 to T8) were identified. Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 7
PL-SIN-EOS-S(4+)-EiP
 
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1+ mentions
RRID:Addgene_21314 Enhanced Green Fluorescence Protein, Puromycin resistance gene Ampicillin PMID:19404254 EGFP and Puro resistance genes driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6626; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 5
Myc-Axin2
 
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1+ mentions
RRID:Addgene_21279 Axin2 Mus musculus Ampicillin PMID:11809808 Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:01 2
pcDNA-FIH1
 
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1+ mentions
RRID:Addgene_21399 HIF1AN Homo sapiens Ampicillin PMID:12615973 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:02 5
PL-SIN-Nanog-EGFP
 
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1+ mentions
RRID:Addgene_21321 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by Nanog promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:01 7
pCS2-MT mouse Axin (Axin MTFu1)
 
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1+ mentions
RRID:Addgene_21287 Axin Mus musculus Ampicillin PMID:9230313 Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:01 3
PL-SIN-EF1α-EGFP
 
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10+ mentions
RRID:Addgene_21320 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by ubiquitous EF1α promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6881; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 13
TRX1 His C32S C35S
 
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1+ mentions
RRID:Addgene_21286 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C32S C35S 2026-08-29 01:02:07 2
TRX1 flag C35S
 
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1+ mentions
RRID:Addgene_21285 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C35S 2026-08-29 01:02:03 4
pBSKS Notch4
 
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1+ mentions
RRID:Addgene_21325 Notch4 Mus musculus Ampicillin PMID:8681805 Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 1
pHR'-ROMK1-CITE-GFP
 
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1+ mentions
RRID:Addgene_21322 ROMK1 Rattus norvegicus Ampicillin PMID:10938328 Backbone Marker:Didier Trono; Backbone Size:10000; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:04 1
TRX1 flag C32S
 
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1+ mentions
RRID:Addgene_21284 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C32S 2026-08-29 01:02:01 3
pHIV-Luciferase
 
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10+ mentions
RRID:Addgene_21375 Ampicillin The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published. NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function. Backbone Size:8713; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:02 32
pHIV-EGFP
 
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50+ mentions
RRID:Addgene_21373 Ampicillin PMID:18371425 HIV-EGFP is a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with EGFP. This construct was described in the publication: Cell Stem Cell. 2008 Jan 10. 2(1):90-102. Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:04 67
M-PKD2 (OF2-3)
 
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1+ mentions
RRID:Addgene_21370 autosomal dominant polycystic kidney disease type II Homo sapiens Ampicillin PMID:11140688 *Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function. Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin wild-type* 2026-08-29 01:02:04 5
pGL3-VEGFR2-225
 
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1+ mentions
RRID:Addgene_21306 VEGFR2 promoter Homo sapiens Ampicillin PMID:19242469 -225 to +268 region of the promoter. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-29 01:02:07 1
pCM1.127
 
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1+ mentions
RRID:Addgene_21384 pie-1 promoter (short, no ATG) in pDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-29 01:02:02 5
pAc5.1C-FLuc-Stop-5BoxB
 
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1+ mentions
RRID:Addgene_21301 5BoxB Ampicillin PMID:16177138 Backbone Marker:Elisa Izaurralde; Backbone Size:7184; Vector Backbone:pAc5.1C-FLuc; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:01 4
CFP-C1-PLCdelta-PH
 
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1+ mentions
RRID:Addgene_21262 PLCdelta Homo sapiens Kanamycin PMID:11134066 Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin PH domain of PLCdelta (human). Also known as PLCdelta-PH-CFP. Lab plasmid WC0026. 2026-08-29 01:02:07 4
Krox20-MSE8 pGL3-TATA
 
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1+ mentions
RRID:Addgene_21261 Krox20-Myelin Specific Enhancer Mus musculus Ampicillin PMID:19179536 Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-29 01:02:03 2

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