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Species: Homo sapiens
Genetic Insert: F-box and WD repeat domain containing 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18381890
Proper citation: RRID:Addgene_19905 Copy
Species: Homo sapiens
Genetic Insert: CUL4A (cullin 4A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12504025
Comments: The CUL4A insert in this plasmid contains a K644R substitution when compared to GenBank reference sequence NM_001008895.
Proper citation: RRID:Addgene_19951 Copy
Species: Homo sapiens
Genetic Insert: CUL4A (cullin 4A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12504025
Proper citation: RRID:Addgene_19953 Copy
Species: Homo sapiens
Genetic Insert: Annexin V
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4606; Vector Backbone:pET12a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8781447
Proper citation: RRID:Addgene_19961 Copy
Species: Homo sapiens
Genetic Insert: cullin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Proper citation: RRID:Addgene_19892 Copy
Species: Homo sapiens
Genetic Insert: cullin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5512; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Comments: Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.
Proper citation: RRID:Addgene_19893 Copy
Species: Homo sapiens
Genetic Insert: cullin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Comments: When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.
Proper citation: RRID:Addgene_19896 Copy
Species: Homo sapiens
Genetic Insert: cullin 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10230407
Proper citation: RRID:Addgene_19895 Copy
Species: Homo sapiens
Genetic Insert: cyclin E2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9840943
Proper citation: RRID:Addgene_19935 Copy
Species: Homo sapiens
Genetic Insert: CDKN1B cyclin-dependent kinase inhibitor 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9840943
Proper citation: RRID:Addgene_19937 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 Y42A/M43A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Comments: flag tag is a variant: YPYDLPDYA
S2A mutation present but does not appear to affect function
Proper citation: RRID:Addgene_19940 Copy
Species: Homo sapiens
Genetic Insert: NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027288
Comments: N51S in seq does not appear to affect function
Proper citation: RRID:Addgene_19943 Copy
Species: Homo sapiens
Genetic Insert: S-phase kinase-associated protein 2 (p45)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9663463
Proper citation: RRID:Addgene_19946 Copy
Species: Homo sapiens
Genetic Insert: CAND1 (cullin-associated and neddylation-dissociated 1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12504025
Proper citation: RRID:Addgene_19948 Copy
Species: Homo sapiens
Genetic Insert: CAND1 (cullin-associated and neddylation-dissociated 1)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12504025
Proper citation: RRID:Addgene_19949 Copy
Species: Homo sapiens
Genetic Insert: cMyc
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18931654
Proper citation: RRID:Addgene_20076 Copy
Species: Homo sapiens
Genetic Insert: cMyc
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5111; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18931654
Comments: Puro resistance cassette was removed by cutting pMSCVpuro with EcoRI and ClaI and religating. EcoRI site was maintained in the vector.
Proper citation: RRID:Addgene_20075 Copy
Species: Homo sapiens
Genetic Insert: hKlf4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5111; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18931654
Comments: Created by Maria J. Barrero.
cDNA was cloned from IMAGE clone
Proper citation: RRID:Addgene_20074 Copy
Species: Homo sapiens
Genetic Insert: hSox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5111; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18931654
Comments: Created by Maria J. Barrero.
cDNA was amplified from ES[4]mRNA.
Proper citation: RRID:Addgene_20073 Copy
Species: Homo sapiens
Genetic Insert: hOct4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5111; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18931654
Comments: Created by Maria J. Barrero.
cDNA was amplified from ES[4]mRNA.
Proper citation: RRID:Addgene_20072 Copy
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