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Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20045 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20049 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20047 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20051 Copy
Species: Homo sapiens
Genetic Insert: Trinucleotide repeat containing 6B
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_20022 Copy
Species: Homo sapiens
Genetic Insert: Trinucleotide repeat containing 6A
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_20021 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20030 Copy
Species: Homo sapiens
Genetic Insert: transcription elongation factor B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5022; Vector Backbone:pcDNA3.1(+)-Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:10878807
Proper citation: RRID:Addgene_20000 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FRB
Proper citation: RRID:Addgene_20225 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20182 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20181 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 2
Vector Backbone Description: Backbone Size:6203; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19150425
Proper citation: RRID:Addgene_20230 Copy
Species: Homo sapiens
Genetic Insert: NRas
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5607; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20205 Copy
Species: Homo sapiens
Genetic Insert: cIAP1 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18846111
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen) and the expression plasmid pMC-cIAP1-FL was constructed by inserting the 3′UTR of cIAP1 downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20209 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19031436
Proper citation: RRID:Addgene_20177 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4500; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19031436
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20176 Copy
Species: Homo sapiens
Genetic Insert: pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
Vector Backbone Description: Backbone Size:0; Vector Backbone:Gateway destination vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18160041
Comments: STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.
Proper citation: RRID:Addgene_20174 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20213 Copy
Species: Homo sapiens
Genetic Insert: C-CrkII nSH3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19031436
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20178 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19139065
Comments: TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
Proper citation: RRID:Addgene_20375 Copy
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