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Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (361-548)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1317 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20mer GFP cassette guide (gGFP) and 5' sgRNA
Vector Backbone Description: Backbone Size:12364; Vector Backbone:pML107 (addgene #67639); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31738765
Proper citation: RRID:Addgene_131784 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20mer CASS5a guide (gCASS5a) and 5' sgRNA
Vector Backbone Description: Backbone Size:12364; Vector Backbone:pML107 (addgene #67639); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31738765
Proper citation: RRID:Addgene_131774 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (1-575)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1318 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GPD promoter-HSP82
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303 (based on pBluescript; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9371781
Comments: Plasmid maintained in DH5alpha.
ClaI-NotI fragment from pTGpd/P82 was cloned into pRS303
Proper citation: RRID:Addgene_1325 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OsTIR1(WT)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74G)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140656 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140657 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COX4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4664; Vector Backbone:pAcGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141153 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Can1-3 sgRNA
Vector Backbone Description: Backbone Size:5500; Vector Backbone:p426; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30683865
Proper citation: RRID:Addgene_145066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mCherry-CaaX
Vector Backbone Description: Backbone Size:1811; Vector Backbone:pGT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139500 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pHSP12 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. The depositor has confirmed that this can happen over time and recommends using more stable bacterial cell strains and performing full plasmid sequencing of these constructs when sub-cloning and transforming to avoid further loss of repeats.
Proper citation: RRID:Addgene_139490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGRE2 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 PP7∆FG-GFPenvy tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEF PP7∆FG-GFPenvy tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPH1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140149 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140151 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GGA2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140150 Copy
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