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Species: Homo sapiens
Genetic Insert: Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGAGATGGGCAGACGGAT-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTCATCATCTTCTCCCTCATC-3'.
Proper citation: RRID:Addgene_16175 Copy
Species: Homo sapiens
Genetic Insert: Polyglutamine binding protein 1
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGCCGCTGCCCGTTGCG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG ATCCTGCTGCTTGGTTCGG-3'.
Proper citation: RRID:Addgene_16174 Copy
Species: Homo sapiens
Genetic Insert: HMG-box transcription factor 1
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGTGTGGGAAGTGAAGAC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG ATGTTGTTGTGAGCCTGAATTG-3'.
Proper citation: RRID:Addgene_16178 Copy
Species: Homo sapiens
Genetic Insert: Quaking
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGTCGGGGAAATGGAAAC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTGCCGGTGGCGGCTCG-3'.
Proper citation: RRID:Addgene_16183 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis-inducing factor
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGTTCCGGTGTGGAGGCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTCTTCATGAATGTTGAATAGTT-3'.
Proper citation: RRID:Addgene_16182 Copy
Species: Homo sapiens
Genetic Insert: PP2A regulatory subunit B, beta isoform
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGAGGAGGACATTGATACC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTAACCTTGTCCTGGAATATA-3'.
Proper citation: RRID:Addgene_16181 Copy
Species: Homo sapiens
Genetic Insert: PRKC gamma
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCTGGTCTGGGCCCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CATGACGGGCACAGGCACT-3'.
Proper citation: RRID:Addgene_16180 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Proper citation: RRID:Addgene_160112 Copy
Species: Mus musculus
Genetic Insert: Ddx58
Vector Backbone Description: Vector Backbone:pENTR223.1 backbone; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160107 Copy
Species: Moraxella bovoculi AAX08_00205
Genetic Insert: Mb2Cas12a RVRR variant
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible Cas12a entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_160327 Copy
Species: Unspecified
Genetic Insert: Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160640 Copy
Species: Synthetic
Genetic Insert: GRLacIBDGal4AD / RDF
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32083668
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160642 Copy
Vector Backbone Description: Backbone Marker:Pasin et al., ACS Synth Biol. 2017;6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354; Backbone Size:3349; Vector Backbone:pLX-B3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28657330
Comments: GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.
Proper citation: RRID:Addgene_160643 Copy
Species: Synthetic
Genetic Insert: P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31034138
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160645 Copy
Species: Unspecified
Genetic Insert: tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pEGB3_omega1; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34276739
Comments: Compatible with GoldenBraid; insert can be released with BsaI.
Proper citation: RRID:Addgene_160646 Copy
Species: Synthetic
Genetic Insert: BASTA / Cas9
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Compatible with GoldenBraid; insert can be released with BsaI.
Proper citation: RRID:Addgene_160647 Copy
Species: V. cholerae
Genetic Insert: VchCAST proteins and crRNA
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33230293
Comments: Previously referred to as INTEGRATE.
Proper citation: RRID:Addgene_160731 Copy
Vector Backbone Description: Vector Backbone:pYPQ141D; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158578 Copy
Species: Synthetic
Genetic Insert: mitoTALEN left and right to target orf125 (orf138)
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31285556
Comments: Tandem expression vector for right and left mitoTALENs to cut kosena-type CMS gene orf125 (also cut Ogura CMS gene orf138)
Proper citation: RRID:Addgene_158736 Copy
Species: Synthetic
Genetic Insert: mitoTALEN left and right to target orf125 (orf138)
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31285556
Comments: Tandem expression vector for right and left mitoTALENs to cut kosena-type CMS gene orf125 (also cut Ogura CMS gene orf138)
Proper citation: RRID:Addgene_158737 Copy
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