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Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9092628
Comments: AUG --> AUU start codon in lacZ
Proper citation: RRID:Addgene_63864 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9092628
Comments: AUG --> AUC start codon in lacZ
Proper citation: RRID:Addgene_63862 Copy
Species: Bacteroides symbiosus
Genetic Insert: pyruvate phosphate dikinase
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19364139
Comments: Written permission was obtained from Prof. Dunaway-Mariano of University of New Mexico, allowing the deposit this plasmid to Addgene.
Pacolyko, D. J., Carroll, L. J., Martin, B. M., Babbitt, P. C., and Dunaway-Mariano, D. (1990) Biochemistry 29, 10757−10765.
Proper citation: RRID:Addgene_64037 Copy
Species: Synthetic
Genetic Insert: PtALSgRNA
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245880 Copy
Species: Synthetic
Genetic Insert: FCY1-UPP
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Proper citation: RRID:Addgene_245873 Copy
Genetic Insert: CsALSgR1.2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Golden Gate entry vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245878 Copy
Vector Backbone Description: Backbone Size:5888; Vector Backbone:pKEK2201; Vector Types:Sucrose counter-selection allelic exchange vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39566662
Proper citation: RRID:Addgene_251701 Copy
Species: Synthetic
Genetic Insert: PylRS-C6a
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pGS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41974833
Comments: Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint.
Proper citation: RRID:Addgene_251554 Copy
Species: Other
Genetic Insert: ABR, lv0, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249981 Copy
Species: Other
Genetic Insert: ABR, lv0*, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250022 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Nova Lifetech Inc.; Backbone Size:10416; Vector Backbone:pRK415; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: This plasmid was constructed via isothermal DNA assembly that was optimized with a proprietary technique protected by US Provisional (US 63/881,962)
Proper citation: RRID:Addgene_252211 Copy
Genetic Insert: CymR inducible systems
Vector Backbone Description: Vector Backbone:mini-CTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: Please visit https://doi.org/10.1101/2025.11.26.690688 for bioRxiv preprint.
Proper citation: RRID:Addgene_253932 Copy
Genetic Insert: CymR inducible systems
Vector Backbone Description: Vector Backbone:mini-CTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: Please visit https://doi.org/10.1101/2025.11.26.690688 for bioRxiv preprint.
Proper citation: RRID:Addgene_253933 Copy
Genetic Insert: sfYFP
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248097 Copy
Genetic Insert: sfCFP
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248098 Copy
Genetic Insert: sfCFP
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248094 Copy
Genetic Insert: tetracycline efflux pump gene
Vector Backbone Description: Backbone Size:3237; Vector Backbone:pK18msB (Addgene plasmid # 177839); Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:42549571
Comments: Contains ≈750 bp homology arms flanking the phaCAB locus in the C. necator H16 genome to replace phaCAB with the tetA tetracycline resistance gene. Please see the annotated map in the Supplementary Documents section.
Proper citation: RRID:Addgene_254853 Copy
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