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Species: Mus musculus
Genetic Insert: metabotropic glutamate receptor 1
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33600762
Comments: Original cDNA from origene (CAT: MR211136)
Proper citation: RRID:Addgene_166227 Copy
Species: Mus musculus
Genetic Insert: VP2C-GFPdeg
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166678 Copy
Species: Mus musculus
Genetic Insert: VP2C-GFPdeg
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166677 Copy
Species: Mus musculus
Genetic Insert: mouse synuclein (from V. Lee)
Vector Backbone Description: Backbone Marker:Virginia Lee; Vector Backbone:pRK172; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33497415
Comments: The original plasmid reference is:
"Selective imaging of internalized proteopathic a-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies" JBC 2017, DOI 0.1074/jbc.M117.780296
Richard J. Karpowicz, Jr. , Conor M. Haney , Tiberiu S. Mihaila , Raizel M. Sandler , E. James Petersson, and Virginia M.-Y. Lee
Proper citation: RRID:Addgene_166671 Copy
Species: Mus musculus
Genetic Insert: β3
Vector Backbone Description: Backbone Marker:SnapGene; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288722
Proper citation: RRID:Addgene_166579 Copy
Species: Mus musculus
Genetic Insert: BimL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166734 Copy
Species: Mus musculus
Genetic Insert: tBid
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166736 Copy
Species: Mus musculus
Genetic Insert: BimEL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166735 Copy
Species: Mus musculus
Genetic Insert: GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166683 Copy
Species: Mus musculus
Genetic Insert: Kitl
Vector Backbone Description: Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30504842
Proper citation: RRID:Addgene_166721 Copy
Species: Mus musculus
Genetic Insert: VP2C-GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166682 Copy
Species: Mus musculus
Genetic Insert: MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166681 Copy
Species: Mus musculus
Genetic Insert: VP2C-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166680 Copy
Species: Mus musculus
Genetic Insert: Bcl-W
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W.
Proper citation: RRID:Addgene_166751 Copy
Species: Mus musculus
Genetic Insert: tBid-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166743 Copy
Species: Mus musculus
Genetic Insert: RGS7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16677 Copy
Species: Mus musculus
Genetic Insert: BimEL-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166742 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166891 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166890 Copy
Species: Mus musculus
Genetic Insert: KIF5A-R280H
Vector Backbone Description: Vector Backbone:pLV-eF1a-IRES::Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166953 Copy
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