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Species: Caenorhabditis elegans
Genetic Insert: Lin-29 b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5442; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52515 Copy
Species: Caenorhabditis elegans
Genetic Insert: Blmp-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5462; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52512 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLG-1
Vector Backbone Description: Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029
Proper citation: RRID:Addgene_65827 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66101 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chronos
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66102 Copy
Species: Caenorhabditis elegans
Genetic Insert: PPTR-1/pLexA-N bait
Vector Backbone Description: Backbone Size:5650; Vector Backbone:pLexA-N Gateway compatible destination vector; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62866 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PPTR-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62868 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-1 S574A
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62877 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-3 T541A S582A T605A
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62878 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEI-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62873 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PAA-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62870 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MBK-2
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62871 Copy
Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-3
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62880 Copy
Species: Caenorhabditis elegans
Genetic Insert: 12aa linker::mNeonGreen::TEV::3xFLAG::12aa linker
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2353; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40463100
Comments: This plasmid contains four unique crRNA sites that can be used for re-editing the initial knock-in and inserting alternate cassettes. See this preprint for details: https://www.biorxiv.org/content/10.1101/2025.05.14.653803v1
Proper citation: RRID:Addgene_253923 Copy
Species: Caenorhabditis elegans
Genetic Insert: 12aa linker::mNeonGreen::TEV::AID*::3xFLAG::12aa linker
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2356; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40463100
Proper citation: RRID:Addgene_253924 Copy
Species: Caenorhabditis elegans
Genetic Insert: SignalPeptide-FLAG-His8-GFP-SID1(D171A)
Vector Backbone Description: Vector Backbone:pT2CAG-MCS-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_253488 Copy
Species: Caenorhabditis elegans
Genetic Insert: cex-1 3'UTR
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The SNPs in pcex-1 do not change the expression pattern of Pcex-1. We observed pattern expression in RIM interneuron only. In addition, the SNP in worm mCherry also does not affect the expression or fluorescence of the RFP.
Proper citation: RRID:Addgene_253828 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3 daf-16::GFP
Vector Backbone Description: Backbone Size:7400; Vector Backbone:MosSci vector (Punc-119-mcs-att ); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
Addgene found the following discrepancies in our result versus the depositor's reference:
2 SNPs in c. Briggsae unc-119(+); one results in premature stop codon Q157STOP
5 SNPs and 3bp indel in daf-16a
Comment from depositor: Functionally, the unc-119 Q7R gene does not affect it to rescue animals with unc-119 mutation. So it should be of no concern. The daf-16a SNPs and indel also do not appear to affect its function, expression or its ability to translocate from cytoplasm to nucleus.
Proper citation: RRID:Addgene_253820 Copy
Species: Caenorhabditis elegans
Genetic Insert: (nlp-51 homology) T2A 3xNLS GFP
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMiniT 2.0; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38021170
Proper citation: RRID:Addgene_227722 Copy
Species: Caenorhabditis elegans
Genetic Insert: T2A 3xNLS GFP with some nlp-51 homology sequence
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMiniT 2.0; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38021170
Proper citation: RRID:Addgene_227721 Copy
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