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Species: Staphylococcus aureus
Genetic Insert: Protein A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32225 Copy
Genetic Insert: QF Kan
Vector Backbone Description: Vector Backbone:pBSC5; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21857163
Comments: Please see http://www.everyvector.com/sequences/show_public/14110 for additional plasmid map.
Proper citation: RRID:Addgene_32321 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of ECFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32436 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the C-terminus of another cDNA by cloning the cDNA into the BamHI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32437 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Comments: For the addition of EYFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
Proper citation: RRID:Addgene_32438 Copy
Species: Gallus gallus
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32791 Copy
Species: Homo sapiens
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32790 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:8157596
Comments: Additional references:
Elhai J, Wolk CP (1988) A versatile class of positive selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138
Kuritz T, Ernst A, Black TA, Wolk CP (1993) High-resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation. Mol Microbiol 8: 101-110
Proper citation: RRID:Addgene_70283 Copy
Species: Homo sapiens
Genetic Insert: neoCV
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27825338
Proper citation: RRID:Addgene_73889 Copy
Species: Synthetic
Genetic Insert: Tol2-kanaR-cryaa-YFP
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26818072
Proper citation: RRID:Addgene_74154 Copy
Species: Synthetic
Genetic Insert: Tol2-kanaR-cryaa-sfGFP
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26818072
Proper citation: RRID:Addgene_74153 Copy
Species: Synthetic
Genetic Insert: Tol2-kanaR-cryaa-dsRed
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26818072
Proper citation: RRID:Addgene_74152 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
Proper citation: RRID:Addgene_71310 Copy
Species: Geobacillus stearothermophilus
Genetic Insert: PheB
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71738 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71737 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68034 Copy
Species: Mus musculus
Genetic Insert: Plexin-B3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68037 Copy
Species: Mus musculus
Genetic Insert: Plexin-B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68036 Copy
Species: Mus musculus
Genetic Insert: Plexin-B1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68035 Copy
Vector Backbone Description: Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29742421
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_128734 Copy
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