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Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35509 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4886; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35456 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4880; Vector Backbone:pRS31x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35455 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6009; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: LEU2 has been modified to remove ClaI, EcoRI and KpnI sites without altering the amino acid sequence of the Leu2 protein.
Proper citation: RRID:Addgene_35454 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4787; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35452 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4788; Vector Backbone:pRS31x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35451 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35566 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17567956
Proper citation: RRID:Addgene_35687 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35565 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35564 Copy
Species: Danio rerio
Genetic Insert: Kdrl-5p-FokI-EL
Vector Backbone Description: Backbone Size:5026; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_35684 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Comments: A G21C point mutation is present in the sequence. The depositing lab notes that this should not alter the function of the plasmid.
Proper citation: RRID:Addgene_35569 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35568 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18971378
Proper citation: RRID:Addgene_35689 Copy
Species: SV40
Genetic Insert: loxP-STOP-loxP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: If you are using the cassette for gene targeting, you should check the number of SV40 repeats in the targeted locus as the lab has noticed that in some ES clones copies of the repeats were missing.
Please also see Lox-Stop-Lox TOPO (http://www.addgene.org/11584/) for a puromycin version of this plasmid and to download a document about using the Lox-Stop-Lox system.
Proper citation: RRID:Addgene_35688 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35562 Copy
Species: Homo sapiens
Genetic Insert: nsa-mir-224
Vector Backbone Description: Backbone Marker:provided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591); Backbone Size:8600; Vector Backbone:pRRL-CMV-PGK-GFP-WPRE (TWEEN); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP.
Proper citation: RRID:Addgene_35683 Copy
Species: Homo sapiens
Genetic Insert: ERbeta
Vector Backbone Description: Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19106194
Proper citation: RRID:Addgene_35561 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35681 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35680 Copy
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