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Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14219); Backbone Size:8004; Vector Backbone:pAG416Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21541367
Proper citation: RRID:Addgene_29621 Copy
Species: Homo sapiens
Genetic Insert: TRPM1
Vector Backbone Description: Backbone Size:9615; Vector Backbone:PLKO (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21109473
Proper citation: RRID:Addgene_29782 Copy
Species: Homo sapiens
Genetic Insert: MSL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20018852
Comments: An A87V mutation is present, it does not effect the function of the plasmid.
Proper citation: RRID:Addgene_29762 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Like Protein 1
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18160654
Proper citation: RRID:Addgene_30141 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein - soluble
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18160654
Proper citation: RRID:Addgene_30147 Copy
Species: Homo sapiens
Genetic Insert: WASP-GBD
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10950951
Proper citation: RRID:Addgene_30113 Copy
Species: Homo sapiens
Genetic Insert: rhVitronectin without deletions
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21478862
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30223 Copy
Species: Homo sapiens
Genetic Insert: rhVitronectin with N and C terminus cleavages
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21478862
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30226 Copy
Species: Homo sapiens
Genetic Insert: IQGAP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9867866
Proper citation: RRID:Addgene_30107 Copy
Species: Homo sapiens
Genetic Insert: GLIS1
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654807
Comments: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation.
IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274
Proper citation: RRID:Addgene_30166 Copy
Species: Homo sapiens
Genetic Insert: PTEN-R11A
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30383 Copy
Species: Homo sapiens
Genetic Insert: PTEN-K6A
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30382 Copy
Species: Homo sapiens
Genetic Insert: PTEN-T383D
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30381 Copy
Species: Homo sapiens
Genetic Insert: PTEN-S10N
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30371 Copy
Species: Homo sapiens
Genetic Insert: PTEN-L186V
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21536651
Proper citation: RRID:Addgene_30379 Copy
Species: Homo sapiens
Genetic Insert: RagD shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against RagD. Target sequence: 5'-CGGCAAGTCGTCTATTCAGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_30321 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26607 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26606 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26608 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26603 Copy
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