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Genetic Insert: ChroME2f-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_171150 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser81Ala mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171223 Copy
Species: Homo sapiens
Genetic Insert: AR-C619Y
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutation occurs at the end of androgen receptor zinc finger 2 and this AR mutant is defective in transactivation but not nuclear import.
Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171222 Copy
Species: Homo sapiens
Genetic Insert: AR-R599A-N600A
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur in the D-box of the androgen receptor Zinc finger 2 that mediates AR dimerization.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171221 Copy
Species: Mus musculus
Genetic Insert: hU6-sgLacZ1-hU6-sgLacZ2
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: Please note Addgene NGS identified two mutations in mCherry (K14E, N210D) and one in KASH domain of Nesprin2 E>G. Depositor confirms plasmid is functional.
Proper citation: RRID:Addgene_171185 Copy
Species: Synthetic
Genetic Insert: Cas12f1
Vector Backbone Description: Backbone Size:8576; Vector Backbone:pCas; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34023220
Proper citation: RRID:Addgene_171182 Copy
Species: Homo sapiens
Genetic Insert: AR-AAFF
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur on the androgen receptor KVFF motif in the P-box of the zinc finger 1 that make contact with DNA. AR mutants of the KVFF motif would lose activity to activate AR-mediated transcription.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171218 Copy
Species: Homo sapiens
Genetic Insert: M-SPOTIT1.1
Vector Backbone Description: Backbone Size:6783; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171216 Copy
Species: Homo sapiens
Genetic Insert: K-SPOTIT1.0
Vector Backbone Description: Backbone Size:6778; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171215 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK041.AAV, pBBR1-KmR_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)_AAV
Proper citation: RRID:Addgene_171175 Copy
Species: Other
Genetic Insert: dTomato
Vector Backbone Description: Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_171174 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22275373
Comments: Flag-tagged human AR Ser-Pro phosphorylation mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171249 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Asp mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171246 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171245 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:6261; Vector Backbone:pPPC020.306; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK134, pBBR1-GmR_J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
Proper citation: RRID:Addgene_171149 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 beta catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171269 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:4577; Vector Backbone:pRK2-KmR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK080, pRK2-KmR_J1-BBa_J23117-mRFP_hAAVS1.
Proper citation: RRID:Addgene_171147 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase and DNA mutagenesis.
Proper citation: RRID:Addgene_171268 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171267 Copy
Species: Synthetic
Genetic Insert: J109 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK058, pBBR1-KmR_J1-mRFP_J109
Proper citation: RRID:Addgene_171145 Copy
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