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Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Constructs for Soft-agar assay
Proper citation: RRID:Addgene_21659 Copy
Species: Homo sapiens
Genetic Insert: Dicer 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Proper citation: RRID:Addgene_21653 Copy
Species: Homo sapiens
Genetic Insert: E2F-1
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1638635
Proper citation: RRID:Addgene_21668 Copy
Species: Homo sapiens
Genetic Insert: DEPTOR PDZ domain
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19446321
Proper citation: RRID:Addgene_21701 Copy
Species: Homo sapiens
Genetic Insert: DEPTOR DEP domains
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19446321
Proper citation: RRID:Addgene_21700 Copy
Species: Homo sapiens
Genetic Insert: Orai1
Vector Backbone Description: Backbone Size:7480; Vector Backbone:MO70; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17293345
Comments: See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).
Proper citation: RRID:Addgene_21662 Copy
Species: Homo sapiens
Genetic Insert: NFAT4
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10497131
Proper citation: RRID:Addgene_21664 Copy
Species: Homo sapiens
Genetic Insert: Atg14L
Vector Backbone Description: Backbone Size:4727; Vector Backbone:pEGFP-C2 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19270696
Proper citation: RRID:Addgene_21635 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation
Proper citation: RRID:Addgene_21639 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722
Proper citation: RRID:Addgene_21753 Copy
Species: Mus musculus
Genetic Insert: myelin protein zero intron1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Proper citation: RRID:Addgene_21630 Copy
Species: M. magnetotacticum
Genetic Insert: magA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11250081
Comments: The sequence inserted into the pCR2.1 vector is larger than just the magA gene. The magA gene is preceded by 373 bp of genomic DNA and followed by 30 bp of genomic DNA. The gene contains 2 amino acid substitutions - S94L and P390S.
The cloned gene was used primarily as a probe. Also see Zurkiya et al. Magn Reson Med. 2008 Jun;59(6):1225-31.
Proper citation: RRID:Addgene_21751 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Proper citation: RRID:Addgene_21645 Copy
Species: Homo sapiens
Genetic Insert: Dicer 1 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Proper citation: RRID:Addgene_21649 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 short UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Proper citation: RRID:Addgene_21644 Copy
Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:British Biotech; Backbone Size:5076; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669
Proper citation: RRID:Addgene_21737 Copy
Species: Homo sapiens
Genetic Insert: Golgi Phosphoprotein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:897; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Invitrogen Gateway Destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553991
Proper citation: RRID:Addgene_21690 Copy
Species: Rattus norvegicus
Genetic Insert: mTOR (1271-2008)
Vector Backbone Description: Backbone Marker:BD Pharmingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12718876
Proper citation: RRID:Addgene_21746 Copy
Species: Drosophila melanogaster
Genetic Insert: mCherry::dMoeABD
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2931; Vector Backbone:pBS Sk-; Vector Types:bacterial propagation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19098902
Proper citation: RRID:Addgene_21744 Copy
Species: Homo sapiens
Genetic Insert: hOCT4 - 2A - hSOX2 - 2A - hKLF4
Vector Backbone Description: Backbone Size:9692; Vector Backbone:pDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19415770
Comments: Plasmid constructed by Kevin Pawlik
Proper citation: RRID:Addgene_21627 Copy
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