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Species: Homo sapiens
Genetic Insert: PP1a
Vector Backbone Description: Backbone Marker:Peti Lab (Addgene #26563); Backbone Size:0; Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18992256
Comments: The backbone is a modified version of pET28 and is also available at Addgene (plasmid #26563)
Proper citation: RRID:Addgene_26566 Copy
Species: Homo sapiens
Genetic Insert: Apoptotic protease activating factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630894
Proper citation: RRID:Addgene_26642 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against Rheb1. Target sequence: 5'-CCTCAGACATACTCCATAGAT-3'.
Proper citation: RRID:Addgene_26625 Copy
Species: Homo sapiens
Genetic Insert: RagB shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against RagB. Target sequence: 5'-CGGGACAACATCTTCCGAAAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_26627 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against Rheb1. Target sequence: 5'-TTATGTTGGTTGGGAATAAGA-3'.
Proper citation: RRID:Addgene_26626 Copy
Species: Homo sapiens
Genetic Insert: p18 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20381137
Comments: shRNA directed against p18. Target sequence: 5'-AGACAGCCAGCAACATCATTG-3'
Proper citation: RRID:Addgene_26631 Copy
Species: Homo sapiens
Genetic Insert: raptor
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20381137
Proper citation: RRID:Addgene_26633 Copy
Species: Homo sapiens
Genetic Insert: STAT3 shRNA
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7200; Vector Backbone:pSIH1-H1-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20197401
Comments: STAT3 shRNA sequence: gatccGCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTg
Proper citation: RRID:Addgene_26596 Copy
Species: Homo sapiens
Genetic Insert: 3x MHC I KB element - luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7707523
Comments: The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).
Proper citation: RRID:Addgene_26699 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20551166
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20729842
Proper citation: RRID:Addgene_26768 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26775 Copy
Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8688416
Comments: There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
Proper citation: RRID:Addgene_26774 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-LIN28A-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26819 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-OCT4-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that OCT4 has some silent mutations.
Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26816 Copy
Species: Homo sapiens
Genetic Insert: dystrophin
Vector Backbone Description: Backbone Size:6656; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral, lentiviral plasmid backbone from L. Naldini; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: Human (alpha) Skeletal Actin (HSA) promotor
Vp intron
Proper citation: RRID:Addgene_26810 Copy
Species: Homo sapiens
Genetic Insert: NICD3
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508304
Comments: EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos
A1757T mutation in Notch3 is not known to affect function.
Proper citation: RRID:Addgene_26894 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26966 Copy
Species: Homo sapiens
Genetic Insert: hChR2(E123T/H134R)-EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20081849
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26968 Copy
Species: Homo sapiens
Genetic Insert: shRNA NEDD4L #1
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa
Proper citation: RRID:Addgene_27016 Copy
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