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http://www.addgene.org/21835

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Comments: The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34.

Proper citation: RRID:Addgene_21835 Copy   

•   Source: Addgene

http://www.addgene.org/21834

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086

Proper citation: RRID:Addgene_21834 Copy   

•   Source: Addgene

http://www.addgene.org/21833

Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V. FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21833 Copy   

•   Source: Addgene

http://www.addgene.org/21832

Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V. FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21832 Copy   

•   Source: Addgene

http://www.addgene.org/21842

Species: L. pneumophila
Genetic Insert: fabI
Vector Backbone Description: Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19578436

Proper citation: RRID:Addgene_21842 Copy   

•   Source: Addgene

http://www.addgene.org/21813

Species: Cricetulus longicaudatus (long-tailed hamster)
Genetic Insert: GADD34 C-term
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Comments: Hamster GADD34 C-term was isolated in a somatic cell genetic screen as a high dose supressor of the intergated stess response. The insert from the retrovirus isolated in that screen was recloned into unique BamH1 and Sal1 sites of pBABEpu and confirmed to suppress the ISR when introduced back into cells.

Proper citation: RRID:Addgene_21813 Copy   

•   Source: Addgene

http://www.addgene.org/21812

Species: Homo sapiens
Genetic Insert: pQE-nTR
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15226295

Proper citation: RRID:Addgene_21812 Copy   

•   Source: Addgene

http://www.addgene.org/21817

Species: Mus musculus
Genetic Insert: PERK (537-1114)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9930704
Comments: Mouse PERK (537-1114), wild-type, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1 Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

Proper citation: RRID:Addgene_21817 Copy   

•   Source: Addgene

http://www.addgene.org/21816

Species: Mus musculus
Genetic Insert: PERK (C-term deleted)
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9930704
Comments: Mouse PERK, C-term deleted (removes amino-acids 582-1081), 9E10-tagged at C-terminus, mammalian expression vector. Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

Proper citation: RRID:Addgene_21816 Copy   

•   Source: Addgene

http://www.addgene.org/21826

Species: simian virus
Genetic Insert: SV40 Large T
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Bluescribe; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: SV40 large T antigen for immortalizing cells. See Author's Map for more information. Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.

Proper citation: RRID:Addgene_21826 Copy   

•   Source: Addgene

http://www.addgene.org/21871

Vector Backbone Description: Backbone Size:3508; Vector Backbone:pMLM36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19798082
Comments: Combinatorial libraries of zinc finger arrays made by OPEN are cloned into this plasmid (see Maeder et al., Mol Cel 2008, PMID 18657511). Ligate zinc finger array fragments with BbsI-digested pBR-UV5-GP-FD2 vector backbone to create plasmids that express the zinc finger array as a FLAG-tagged Gal11P fusion in the B2H system. The lacUV5 promoter which drives expression of the Gal11P fusion protein contains a lac operator and therefore can be repressed by lac repressor. This plasmid is a low copy number plasmid and therefore we recommend that minipreps be performed from a 4 to 10 ml culture. Annotations for pMLM36: 12-17: lacUV5 promoter -35 box / 36-41: lacUV5 promoter -10 box / 48: lacUV5 promoter transcription start point / 86-88: Start codon for Gal11P protein fragment / 89-358: Coding sequence for Gal11P a.a. 263-352 (with additional translationally silent mutations) / 407-412: BbsI site #1 / 433-438: BbsI site #2 / 568-1024: f1 phage origin of replication / 1156-2013: beta-lactamase gene cassette (confers resistance to ampicillin and carbenicillin) / 2016-2956: ColE1 origin of replication (plasmid origin) / 3203-3390: rop gene (associated with regulation of copy number)

Proper citation: RRID:Addgene_21871 Copy   

•   Source: Addgene

http://www.addgene.org/21873

Vector Backbone Description: Backbone Size:5725; Vector Backbone:pMLM292; Vector Types:Mammalian Expression, zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20010934
Comments: Zinc fingers are cloned in between the unique XbaI and BamHI sites. This results in an expression vector which encodes a ZFN with the OPEN zinc finger array fused to the mutant FokI nuclease domain. pMLM292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains 235- 822: CMV promoter / 771-775: CAAT box / 804-808: TATA box / 819: 3' end of hCMV / 904-906: ZFN start codon / 910-930: SV40 nuclease localization signal (NLS) / 940- 963: FLAG tag / 964- 969: XbaI site / 980- 985: BamHI site / 986-1573: FokI nuclease domain / 1292&1294: Q486E heterodimer FokI mutation / 1331: I499L heterodimer FokI mutation / 1574-1576: ZFN stop codon / 1686-1910: BGH polyadenylation sequence / 1956-2384: f1 origin / 2411-2720: SV40 early promoter and origin / 2775-2841: EM-7 Promoter / 2842-3240: Blasticidin resistance gene / 3398-3528: SV40 late polyA signal / 3955-4574: pUC origin / 4729-5589: ampicillin (bla) resistance gene (complementary strand) / 5590-5688: bla promoter (complementary strand)

Proper citation: RRID:Addgene_21873 Copy   

•   Source: Addgene

http://www.addgene.org/21761

Genetic Insert: ECFP -EYFP linked by flexible linker containing two SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17073440

Proper citation: RRID:Addgene_21761 Copy   

•   Source: Addgene

http://www.addgene.org/21880

Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3.

Proper citation: RRID:Addgene_21880 Copy   

•   Source: Addgene

http://www.addgene.org/21853

Species: Mus musculus
Genetic Insert: mCD36
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)

Proper citation: RRID:Addgene_21853 Copy   

•   Source: Addgene

http://www.addgene.org/21852

Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)

Proper citation: RRID:Addgene_21852 Copy   

•   Source: Addgene

http://www.addgene.org/21850

Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11106749
Comments: Addgene sequencing results show 2 mismatches with author's sequence and NCBI sequence for ATF4. Mouse ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter in a pGL3 backbone TK.mATF4.UTR.Luc.pGL3 (5202nt, predicted). TK promoter: 58..184; mATF4 5'UTR and AUG: 185..470; luciferase coding sequence: 472..2121; poly A signals: 2156..2377

Proper citation: RRID:Addgene_21850 Copy   

•   Source: Addgene

http://www.addgene.org/21867

Species: Mesoplasma florum
Genetic Insert: Lon
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18852454
Comments: A detailed protocol for purification of mf-lon can be found in the associated paper.

Proper citation: RRID:Addgene_21867 Copy   

•   Source: Addgene

http://www.addgene.org/179440

Species: Homo sapiens
Genetic Insert: H2B-mIFP-T2A-rTetR-HDAC4
Vector Backbone Description: Vector Backbone:pPB (PiggyBac); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_179440 Copy   

•   Source: Addgene

http://www.addgene.org/179435

Species: Homo sapiens
Genetic Insert: TRE-cit-coreHS4-mch (SC)
Vector Backbone Description: Vector Backbone:pAAVS1 donor ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_179435 Copy   

•   Source: Addgene