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Species: Mus musculus
Genetic Insert: EB3-GFP-6xHis
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30262468
Proper citation: RRID:Addgene_130983 Copy
Species: Mus musculus
Genetic Insert: EB1-mCherry-6xHis
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30262468
Proper citation: RRID:Addgene_130984 Copy
Species: Mus musculus
Genetic Insert: EB2-GFP-6xHis
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30262468
Proper citation: RRID:Addgene_130982 Copy
Species: Mus musculus
Genetic Insert: TCRB
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/466243v5 for bioRxiv preprint.
Proper citation: RRID:Addgene_131034 Copy
Species: Mus musculus
Genetic Insert: Trib3
Vector Backbone Description: Backbone Marker:Warren Pear (Addgene plasmid # 27490); Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20410507
Comments: Trib3 contains a N201T mismatch located within the pseudokinase domain but out-with core motif.
Both Asparagine and Threonine are polar and form hydrogen bonds as both donors and acceptors; substitution should have minimal effect on protein structure/function.
Proper citation: RRID:Addgene_131159 Copy
Species: Mus musculus
Genetic Insert: TCRB
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/466243v5 for bioRxiv preprint.
Proper citation: RRID:Addgene_131036 Copy
Species: Mus musculus
Genetic Insert: Trib3
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20410507
Comments: Murine Trib3 with an N-terminal Flag tag cloned into BamHI-EcoRI site (with Bgl2 so BamHI site is destroyed). Release insert with HindIII and XhoI.
Proper citation: RRID:Addgene_131157 Copy
Species: Mus musculus
Genetic Insert: Trib1
Vector Backbone Description: Backbone Marker:Warren Pear (Addgene plasmid # 27490); Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20410507
Proper citation: RRID:Addgene_131158 Copy
Species: Mus musculus
Genetic Insert: Trib2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17097562
Proper citation: RRID:Addgene_131155 Copy
Species: Mus musculus
Genetic Insert: Trib1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20410507
Proper citation: RRID:Addgene_131156 Copy
Species: Mus musculus
Genetic Insert: Trib2
Vector Backbone Description: Backbone Marker:Warren Pear (Addgene plasmid # 27490); Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17097562
Comments: PCR from pHMA FL Trib2, N term: 1-145 amino acids = 16 kDa; start-Myc-XhoI-N term-stop-EcoRI
Proper citation: RRID:Addgene_131153 Copy
Species: Mus musculus
Genetic Insert: Trib2
Vector Backbone Description: Backbone Marker:Warren Pear (Addgene plasmid # 27490); Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17097562
Comments: Murine Trib2 (NM_144551.5); XhoI-ATG-Flag-Trib2-EcoRI
Proper citation: RRID:Addgene_131154 Copy
Species: Mus musculus
Genetic Insert: -2.6kb - 0 of mTrib2 upstream of gene transcriptional start site
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24516045
Comments: The insert contains a A1815T mismatch. This is within a putative CTTTGCAAT CEBP binding site which has not been experimentally validated, and without experimentally validated E2F binding sites.
Proper citation: RRID:Addgene_131151 Copy
Species: Mus musculus
Genetic Insert: i-WT-r.Donor
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:3500; Vector Backbone:pCR™-Blunt vector; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131324 Copy
Species: Mus musculus
Genetic Insert: Y365A or A365Y inducible rescue
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131323 Copy
Species: Mus musculus
Genetic Insert: EED-KO-gRNA-1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131321 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9257; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131477 Copy
Species: Mus musculus
Genetic Insert: Caspase-1 (C285A)
Vector Backbone Description: Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30853218
Proper citation: RRID:Addgene_131353 Copy
Species: Mus musculus
Genetic Insert: Caspase-1 (C285A)-NpLxIS
Vector Backbone Description: Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30853218
Proper citation: RRID:Addgene_131354 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9262; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131475 Copy
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