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Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
Vector Backbone Description: Vector Backbone:pJYS1Ptac; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37348112
Comments: Plasmid can also be transformed in Corynebacterium glutamicum, grown at 30 C
Proper citation: RRID:Addgene_203816 Copy
Species: Mus musculus
Genetic Insert: trLAToC27AC30A
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203748 Copy
Species: Homo sapiens
Genetic Insert: trLATm1 (PtoL)
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203749 Copy
Species: Mus musculus
Genetic Insert: trLAToC27A
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203747 Copy
Species: Homo sapiens
Genetic Insert: Lyn n-term
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203755 Copy
Species: Homo sapiens
Genetic Insert: trCD45
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203753 Copy
Species: Homo sapiens
Genetic Insert: Src15 n-term
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203754 Copy
Species: Homo sapiens
Genetic Insert: trCD4o
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203751 Copy
Species: Homo sapiens
Genetic Insert: trLATm2(allL+A)
Vector Backbone Description: Backbone Marker:Addgene; Michael Davidson's lab; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203750 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - SmallBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Comments: This plasmid also contains the coding sequence for the LargeBiT fragment of the NanoBiT split NanoLuc luciferase downstream of the SmallBiT coding sequence, but there is a stopcodon after the strep-tag fused to SmallBiT. Hence, this plasmid encodes for the SpdCas9-SmallBiT protein by default. By cleaving the plasmid with SpeI restriction enzyme, the SmallBiT part may be removed, and after selfligation the plasmid then encodes for the SpCas9-LargeBiT protein (i.e. plasmid #198751 is obtained).
Proper citation: RRID:Addgene_198750 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - LargeBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Proper citation: RRID:Addgene_198751 Copy
Species: Synthetic
Genetic Insert: p15A ORI plus NGS barcode 24
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199054 Copy
Species: Synthetic
Genetic Insert: pBC1 ORI plus NGS barcode 25
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199055 Copy
Species: Synthetic
Genetic Insert: pNG2 ORI plus NGS barcode 22
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199052 Copy
Species: Synthetic
Genetic Insert: pSG5 ORI plus NGS barcode 20
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199050 Copy
Species: Synthetic
Genetic Insert: γω assembly linker
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199058 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199065 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.
Proper citation: RRID:Addgene_199067 Copy
Species: Synthetic
Genetic Insert: pIP501 ORI plus NGS barcode 4
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199036 Copy
Species: Synthetic
Genetic Insert: pUB110 ORI plus NGS barcode 2
Vector Backbone Description: Vector Backbone:pGLDV_5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_199034 Copy
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