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Species: Mus musculus
Genetic Insert: ALK6
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26701726
Proper citation: RRID:Addgene_80883 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-CT
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_80953 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT 4A
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_80952 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_80951 Copy
Species: Mus musculus
Genetic Insert: TDG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26932196
Proper citation: RRID:Addgene_81050 Copy
Species: Mus musculus
Genetic Insert: TDG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26932196
Proper citation: RRID:Addgene_81051 Copy
Species: Mus musculus
Genetic Insert: TET1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3780; Vector Backbone:pCDF-Duet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:26932196
Proper citation: RRID:Addgene_81052 Copy
Species: Mus musculus
Genetic Insert: Rheb shRNA
Vector Backbone Description: Backbone Marker:Jacks Lab (MIT); Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24139800
Comments: pSico allows for conditional (Cre-Lox), stable expression of shRheb. Cre expression excises GFP and allows for shRheb expression.
Proper citation: RRID:Addgene_81087 Copy
Species: Mus musculus
Genetic Insert: eIF4A1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV IRES GFP (MIG); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25079319
Proper citation: RRID:Addgene_70042 Copy
Species: Mus musculus
Genetic Insert: mouse inhibitor of carbonic anhydrase
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pNUT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17511619
Proper citation: RRID:Addgene_70162 Copy
Species: Mus musculus
Genetic Insert: eIF4A1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV IRES GFP (MIG); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25079319
Proper citation: RRID:Addgene_70044 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Tuschl lab, Addgene plasmid #70071; Backbone Size:10748; Vector Backbone:pLVE-FH-IN; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Proper citation: RRID:Addgene_70072 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Size:11522; Vector Backbone:CS-TRE-mOKS-PRE-Ubc-rTTA-I2G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: RNP1 of RRM domain is mutated (Y102A).
Silent mutations are introduced to escape from RNAi using pLKO.1-shDnd1-No. 1,6, and 8 deposited in Addgene (plasmids #70058, 70067, and 70069).
Proper citation: RRID:Addgene_70075 Copy
Species: Mus musculus
Genetic Insert: lyso-pHoenix
Vector Backbone Description: Backbone Size:3878; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26551543
Proper citation: RRID:Addgene_70112 Copy
Species: Mus musculus
Genetic Insert: lyso-pHluorin
Vector Backbone Description: Backbone Size:3878; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26551543
Proper citation: RRID:Addgene_70113 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000217928
Proper citation: RRID:Addgene_70060 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000257609
Proper citation: RRID:Addgene_70066 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000191705
Proper citation: RRID:Addgene_70065 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000247049
Proper citation: RRID:Addgene_70069 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: *modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins.
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_70129 Copy
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