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Species: Mus musculus
Genetic Insert: Forkhead box A2
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:36686; Vector Backbone:pAd/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_131581 Copy
Species: Mus musculus
Genetic Insert: Mitogen-activated protein kinase phosphatase 3
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:36686; Vector Backbone:pAd/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_131579 Copy
Species: Mus musculus
Genetic Insert: Lyl1
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131612 Copy
Species: Mus musculus
Genetic Insert: Etv3
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131610 Copy
Species: Mus musculus
Genetic Insert: Gfi1b
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131611 Copy
Species: Mus musculus
Genetic Insert: Meis1
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131605 Copy
Species: Mus musculus
Genetic Insert: Trib3
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131606 Copy
Species: Mus musculus
Genetic Insert: Hhex
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770078
Proper citation: RRID:Addgene_131600 Copy
Species: Mus musculus
Genetic Insert: CNP sgRNA
Vector Backbone Description: Vector Backbone:PB-puro; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30355738
Proper citation: RRID:Addgene_126610 Copy
Species: Mus musculus
Genetic Insert: tRNA 2' phosphotransferase 1
Vector Backbone Description: Vector Backbone:PB-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30355738
Proper citation: RRID:Addgene_126607 Copy
Species: Mus musculus
Genetic Insert: 2'3' cyclic nucleotide phosphodiesterase
Vector Backbone Description: Vector Backbone:PB-bsd; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30355738
Comments: Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.
Proper citation: RRID:Addgene_126608 Copy
Species: Mus musculus
Genetic Insert: Lrig1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Mice available from the MMRRC (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=69516).
Proper citation: RRID:Addgene_126651 Copy
Species: Mus musculus
Genetic Insert: Ighg2b
Vector Backbone Description: Backbone Size:5729; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33268892
Comments: ENA accession: LR588430, https://www.ebi.ac.uk/ena/browser/view/LR588430
Proper citation: RRID:Addgene_127155 Copy
Species: Mus musculus
Genetic Insert: N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Entry vector with transgene for gateway cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127177 Copy
Species: Mus musculus
Genetic Insert: Mouse Cdk12 Intron 3 sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang Lab (Addgene Plasmid #42230); Backbone Size:8484; Vector Backbone:pX330; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127174 Copy
Species: Mus musculus
Genetic Insert: Mouse Cyclin Dependent Kinase 12
Vector Backbone Description: Backbone Marker:Albert Cheng (Jackson Labs); Backbone Size:10369; Vector Backbone:PBNeo-TetO-Dest; Vector Types:Mammalian Expression, Piggybac Transposase Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127178 Copy
Species: Mus musculus
Genetic Insert: Myc (NM_001177352)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:7450; Vector Backbone:pEZ-M56; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: based on published work for human
Proper citation: RRID:Addgene_127286 Copy
Species: Mus musculus
Genetic Insert: Myc (NM_001177352)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:7450; Vector Backbone:pEZ-M56; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: based on published work for human
Proper citation: RRID:Addgene_127285 Copy
Species: Mus musculus
Genetic Insert: ELL3 (NM_145973)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:8565; Vector Backbone:pEZ-Lv225; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127283 Copy
Species: Mus musculus
Genetic Insert: ELL1 (NM_007924 plus CEYLHNK to AEYLHNK)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:8565; Vector Backbone:pEZ-Lv225; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Fails to degrade myc as published for human
Proper citation: RRID:Addgene_127279 Copy
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