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Species: Mus musculus
Genetic Insert: Promoter+V-region of IgH of A20 B cell line
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18025212
Comments: The sequence is show in Figure 2 of the associated publication.
Proper citation: RRID:Addgene_127249 Copy
Species: Mus musculus
Genetic Insert: IgG2b gene with exons and alt pAs
Vector Backbone Description: Vector Backbone:pSV2gpt; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749764
Comments: Contains the whole 11 kb IgG2b gene from MPC11
Proper citation: RRID:Addgene_127248 Copy
Species: Mus musculus
Genetic Insert: BMP7
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:7941; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32117236
Proper citation: RRID:Addgene_127375 Copy
Species: Mus musculus
Genetic Insert: ROSA26
Vector Backbone Description: Backbone Marker:Feng Zhang laboratory; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32117236
Proper citation: RRID:Addgene_127376 Copy
Species: Mus musculus
Genetic Insert: Stat3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6150; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31221664
Comments: A662C and N664C. Residues changed to Cys renders the molecule constitutively active (Addgene #8722).
Proper citation: RRID:Addgene_127540 Copy
Species: Mus musculus
Genetic Insert: Nanog
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6149; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31221664
Comments: This plasmid codes for the activation domain of mouse Nanog (last 110 a.a).
Proper citation: RRID:Addgene_127541 Copy
Species: Mus musculus
Genetic Insert: Klf2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5422; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31221664
Proper citation: RRID:Addgene_127539 Copy
Species: Mus musculus
Genetic Insert: TRAF3ip3
Vector Backbone Description: Vector Backbone:pcDNA-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Depositor confirms these mutations from NCBI reference NP_694777.3 do not affect function.
Proper citation: RRID:Addgene_125181 Copy
Species: Mus musculus
Genetic Insert: Tbkbp1
Vector Backbone Description: Backbone Marker:Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557; Vector Backbone:pCLXSN(GFP)-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022064
Proper citation: RRID:Addgene_125179 Copy
Species: Mus musculus
Genetic Insert: Tbkbp1
Vector Backbone Description: Backbone Marker:Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557; Vector Backbone:pCLXSN(GFP)-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022064
Proper citation: RRID:Addgene_125177 Copy
Species: Mus musculus
Genetic Insert: PSDd1.2-GFP
Vector Backbone Description: Vector Backbone:pCAFNF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37290446
Comments: PSDd1.2-GFP was described in PMID: 10402201.
Arnold, D. B. & Clapham, D. E.Molecular determinants for subcellular localizationof PSD-95 with an interacting K1 channel. Neuron 23, 149–157 (1999).
Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_125581 Copy
Species: Mus musculus
Genetic Insert: mini-nesprin-2G
Vector Backbone Description: Backbone Marker:Invitroogen; Backbone Size:5400; Vector Backbone:pcdna 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31719915
Proper citation: RRID:Addgene_125555 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein beta LIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). This plasmid is designed to express the LIP isoform of the protein.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12561 Copy
Species: Mus musculus
Genetic Insert: Lis1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15473966
Proper citation: RRID:Addgene_12574 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein delta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16632469
Comments: When this C/EBPdelta construct was created, an NcoI restriction site was introduced that overlaps the start (AUG) codon to produce an optimal translation initiation site. This cloning strategy resulted in a S2G mutation in C/EBPdelta as a cloning "scar."
Proper citation: RRID:Addgene_12559 Copy
Species: Mus musculus
Genetic Insert: RLF-CAAX
Vector Backbone Description: Backbone Size:5137; Vector Backbone:pBabe HAII; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12592 Copy
Species: Mus musculus
Genetic Insert: GB1 domain of protein G as N terminal tag, with Bcl2 as insert
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9385638
Comments: In GEV1, a noncleavable linker (Pro-Gly-Gly-Pro-Ala-Ser) is encoded between the GBl domain and the N-terminus of the protein of interest. Genes can be inserted into this vector using Nhe I and Xho I cloning sites, and, if no stop codons are introduced at the 5' end of an insert, the vector encodes a C-terminal poly His-tag.
Also see: "A rapid method to attain isotope labeled small soluble peptides for NMR studies", Journal of Biomolecular NMR, Vol 26, No 3, July 2003.
Proper citation: RRID:Addgene_12615 Copy
Species: Mus musculus
Genetic Insert: L7-6, Purkinje cell specific promoter
Vector Backbone Description: Backbone Size:7914; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828391
Comments: Please note that the Addgene verified sequence contains a truncated ITR compared to the depositor's reference sequence. This truncation was present in the original stock and still worked well for AAV packaging.
Proper citation: RRID:Addgene_126462 Copy
Species: Mus musculus
Genetic Insert: Fndc3b
Vector Backbone Description: Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541092
Proper citation: RRID:Addgene_126443 Copy
Species: Mus musculus
Genetic Insert: BMP-RIB
Vector Backbone Description: Backbone Size:6600; Vector Backbone:LPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12379805
Comments: LPCX was made by replacing the neo gene of LNCX with the puro gene.
Proper citation: RRID:Addgene_12642 Copy
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