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Species: Mus musculus
Genetic Insert: Tfeb
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26944679
Comments: Depositor states that numbering of mutations may vary depending on the isoform in question and confirms that this plasmid should function as described.
Proper citation: RRID:Addgene_79014 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse M33 cDNA, C-terminal flag. (kingston #1391)
Proper citation: RRID:Addgene_1993 Copy
Species: Mus musculus
Genetic Insert: Ring1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse RING1A cDNA, untagged. (kingston #1392)
Proper citation: RRID:Addgene_1994 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18450484
Proper citation: RRID:Addgene_19988 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18450484
Proper citation: RRID:Addgene_19987 Copy
Species: Mus musculus
Genetic Insert: Il12b promoter
Vector Backbone Description: Backbone Size:5598; Vector Backbone:pGL2B; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9234715
Comments: Luciferase Reporter driven by 400 base pair promoter of the Il12b gene.
Proper citation: RRID:Addgene_20020 Copy
Species: Mus musculus
Genetic Insert: Nfkb1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20018 Copy
Species: Mus musculus
Genetic Insert: c-Rel
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20016 Copy
Species: Mus musculus
Genetic Insert: p21 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978791
Proper citation: RRID:Addgene_20014 Copy
Species: Mus musculus
Genetic Insert: c-Rel
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166378
Comments: See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Note from depositing lab: c-Rel is expressed at a lower level than other NF-kB family members.
Proper citation: RRID:Addgene_20013 Copy
Species: Mus musculus
Genetic Insert: Notch 4 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD4 utilized HindIII/XbaI sites. NICD4 (Val-1463 to Asn-1964 of full length mouse N4) was amplified from the Int-3 plasmid (gift from Dr. J. Kitajewski) using primers 5HinN4 (5'-GGCCAAGCTTGTCCTCCAGCTCATTCGGCGACGG-3') and 3XbaN4
(5'-GCTCTAGACGGCTTCCGGTGCCTAGTTCAG-3').
Proper citation: RRID:Addgene_20186 Copy
Species: Mus musculus
Genetic Insert: Notch 1 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD1 was cloned into XbaI/BamHI sites. NICD1 (Val-1744 to Lys-2531 of full-length mouse N1) was amplified using primers 5XbaN1 (5'-GCCTCTAGAGTGCTGCTGTCCCGCAAGCGC-3') and 3BamN1 (5'-CGGGATCCTTATTTAAATGCCTCTGGAATGTGGG-3').
Proper citation: RRID:Addgene_20183 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS1
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20238 Copy
Species: Mus musculus
Genetic Insert: CFP-FKBP-PIPK (D253A)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16990515
Proper citation: RRID:Addgene_20158 Copy
Species: Mus musculus
Genetic Insert: CFP-FKBP-PIPK
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16990515
Proper citation: RRID:Addgene_20157 Copy
Species: Mus musculus
Genetic Insert: mIL-17
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18849990
Proper citation: RRID:Addgene_20128 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS3
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20242 Copy
Species: Mus musculus
Genetic Insert: mIL-17
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18849990
Proper citation: RRID:Addgene_20126 Copy
Species: Mus musculus
Genetic Insert: mIL-17
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18849990
Proper citation: RRID:Addgene_20125 Copy
Species: Mus musculus
Genetic Insert: mIL-17
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18849990
Proper citation: RRID:Addgene_20124 Copy
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