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Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180701 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180706 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180703 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180704 Copy
Species: Other
Genetic Insert: Portal
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188833 Copy
Species: Synthetic
Genetic Insert: S/1 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180710 Copy
Species: Synthetic
Genetic Insert: 1/2 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180712 Copy
Species: Synthetic
Genetic Insert: 5/E Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180719 Copy
Species: Other
Genetic Insert: Terminase
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188837 Copy
Species: Other
Genetic Insert: Terminase
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188838 Copy
Species: Synthetic
Genetic Insert: 2/E Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180713 Copy
Species: Synthetic
Genetic Insert: 2/3 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180714 Copy
Species: Synthetic
Genetic Insert: 3/4 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180716 Copy
Species: Synthetic
Genetic Insert: tdTomato sgRNA
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8652; Vector Backbone:PB_rtTA_BsmBI; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BsmBI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_188488 Copy
Species: K. rhaeticus
Genetic Insert: K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180534 Copy
Species: Synthetic
Genetic Insert: Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162522 Copy
Species: Synthetic
Genetic Insert: Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162525 Copy
Species: Synthetic
Genetic Insert: Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162524 Copy
Species: Other
Genetic Insert: Forsur-6
Vector Backbone Description: Backbone Size:2840; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35951700
Proper citation: RRID:Addgene_188860 Copy
Species: Homo sapiens
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36791199
Proper citation: RRID:Addgene_188101 Copy
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