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Species: Drosophila melanogaster
Genetic Insert: CG1845
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34481 Copy
Species: Drosophila melanogaster
Genetic Insert: CG6604
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34406 Copy
Species: Drosophila melanogaster
Genetic Insert: CG9045
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34405 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report-LUC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Comments: This is a translation reporter. KLF4 cDNA (an 306 bp EcoRI fragment derived from the KLF4 3' UTR) was cloned into the PmeI site in the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at KLF4-internal EcoRI sites within the KLF4 3' UTR, followed by Klenow treatment.
Proper citation: RRID:Addgene_34598 Copy
Vector Backbone Description: Backbone Marker:LT Chow/UAB; Backbone Size:6600; Vector Backbone:pLJD; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21518959
Comments: doi:10.1101/gad.9.19.2335
Genes Dev. 1995 9: 2335-2349
S Cheng, D C Schmidt-Grimminger, T Murant, et al.
Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes.
Proper citation: RRID:Addgene_34595 Copy
Species: Drosophila melanogaster
Genetic Insert: CG9495
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34514 Copy
Species: Drosophila melanogaster
Genetic Insert: CG8013
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34513 Copy
Species: Drosophila melanogaster
Genetic Insert: CG1070
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34511 Copy
Species: Drosophila melanogaster
Genetic Insert: CG6701
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34518 Copy
Species: Drosophila melanogaster
Genetic Insert: CG5249
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34517 Copy
Species: Drosophila melanogaster
Genetic Insert: CG2714
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34516 Copy
Species: Drosophila melanogaster
Genetic Insert: CG9776
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34519 Copy
Genetic Insert: NLS-LSS-mKate2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20212155
Comments: There is an 18bp deletion in Addgene's sequencing results relative to the full plasmid sequence (at position 2174-2192bp). This region is immediately after the LSS-mKate2 gene and is not critical for plasmid performance.
There is also a single nucleotide mismatch that results in an F22S change in the linker between the NLS and LSS-mKate2. It should not have any effect on the fusion performance.
Two other mismatches do not alter amino acid sequence.
Proper citation: RRID:Addgene_34586 Copy
Genetic Insert: tRNA^Sep
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:21868676
Proper citation: RRID:Addgene_34624 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_34623 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_34589 Copy
Species: Mus musculus
Genetic Insert: Arg1
Vector Backbone Description: Vector Backbone:pMIY; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34580 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22053050
Proper citation: RRID:Addgene_34611 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19915540
Comments: MG1655 for expression
Proper citation: RRID:Addgene_34617 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMSCVhygro; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368440
Proper citation: RRID:Addgene_34565 Copy
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