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Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178728 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178729 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178723 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKS4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178724 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178722 Copy
Species: Synthetic
Genetic Insert: Nuclease-dead Lb Cas12a
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35414015
Proper citation: RRID:Addgene_183955 Copy
Species: Synthetic
Genetic Insert: EBFP2-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184461 Copy
Species: Synthetic
Genetic Insert: TagRFP-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184449 Copy
Species: Synthetic
Genetic Insert: TagRFP-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184447 Copy
Species: Synthetic
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179887 Copy
Species: Synthetic
Genetic Insert: iRFP702-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184455 Copy
Species: Synthetic
Genetic Insert: mCardinal-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184453 Copy
Species: Synthetic
Genetic Insert: LiLac fluorescent sensor of lactate
Vector Backbone Description: Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35614105
Comments: mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.)
Proper citation: RRID:Addgene_184570 Copy
Species: Synthetic
Genetic Insert: gRNA TBP-based CIRTS
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31230714
Proper citation: RRID:Addgene_132547 Copy
Species: Synthetic
Genetic Insert: GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183402 Copy
Species: Synthetic
Genetic Insert: LgBiT-FRB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183403 Copy
Species: Synthetic
Genetic Insert: GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183400 Copy
Species: Synthetic
Genetic Insert: iRFP702-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184456 Copy
Species: Synthetic
Genetic Insert: LgBiT-CaM
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183401 Copy
Species: Synthetic
Genetic Insert: iRFP702-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184457 Copy
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