Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-sGFP1-10
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38866742
Proper citation: RRID:Addgene_199475 Copy
Species: Synthetic
Genetic Insert: Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38866742
Proper citation: RRID:Addgene_199489 Copy
Species: Synthetic
Genetic Insert: Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38866742
Proper citation: RRID:Addgene_199487 Copy
Species: Synthetic
Genetic Insert: Gal4vp16/4xnrUAS-mTagBFP2
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38866742
Proper citation: RRID:Addgene_199486 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40A5-EGFP-6PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG044/P297
Proper citation: RRID:Addgene_201351 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40A4-EGFP-4PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG060/P313
Proper citation: RRID:Addgene_201360 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40-EGFP
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG063/P523
Proper citation: RRID:Addgene_201363 Copy
Species: Synthetic
Genetic Insert: tagRFPt
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
A R102C mutation was found in puromycin that does not affect plasmid function.
Proper citation: RRID:Addgene_201089 Copy
Species: Synthetic
Genetic Insert: mScaret-I
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
A R102C mutation was found in puromycin that does not affect plasmid function.
Proper citation: RRID:Addgene_201093 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40-EGFP-4PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG072/P532
Proper citation: RRID:Addgene_201371 Copy
Species: Synthetic
Genetic Insert: Ppyr9eh
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
Proper citation: RRID:Addgene_201443 Copy
Species: Synthetic
Genetic Insert: Gal4-VP64-IRES-mCherry
Vector Backbone Description: Backbone Size:8953; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37268649
Proper citation: RRID:Addgene_203909 Copy
Species: Synthetic
Genetic Insert: ePPE,Cre
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37095350
Proper citation: RRID:Addgene_201739 Copy
Species: Synthetic
Genetic Insert: SpCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65777 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
UPDATE 5/31/2016: The Joung Lab recommends using BPK2660 (Addgene plasmid # 70709) instead of VVT1 as an SaCas9 guide RNA cloning vector, as guide RNAs cloned into BPK2660 are more effective than those cloned into VVT1. The difference in efficacy is due to VVT1 containing the full length guide RNA (predicted from the natural CRISPR sequence in S. aureus), whereas BPK2660 uses a truncated gRNA that is more effective. For the same oligo/spacer, the rate of mutagenesis is greater using BPK2660. See Kleinstiver et al., 2015 Nat Biotech for additional information on the truncated gRNA plasmid (https://www.ncbi.nlm.nih.gov/pubmed/26524662).
Proper citation: RRID:Addgene_65779 Copy
Species: Synthetic
Genetic Insert: gltA2-udhA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65819 Copy
Species: Synthetic
Genetic Insert: udhA guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65818 Copy
Species: Synthetic
Genetic Insert: zwf guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65825 Copy
Species: Synthetic
Genetic Insert: proD guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65820 Copy
Species: Synthetic
Genetic Insert: V5-human TEV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20308068
Proper citation: RRID:Addgene_65800 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
