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Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B1;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34661 Copy
Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110587
Comments: 3 amino acids, GSM, were introduced after aa148 for cloning purposes.
Proper citation: RRID:Addgene_34701 Copy
Species: Homo sapiens
Genetic Insert: HORMA domain containing protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34821 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037171
Proper citation: RRID:Addgene_34705 Copy
Species: Homo sapiens
Genetic Insert: epithelial stromal interaction 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34826 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Created by Köster and Fraser; Vector Backbone:act-GVP-UG; Vector Types:Xenopus expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22113462
Comments: The Sox2/Oct3-4 enhancer elements and a minimal promoter from the murine FGF4 were subcloned from the plasmid described in Figure 1 of Ambrosetti et al. (2000). The fragment contains six tandem repeats of the 35-bp fragment containing the Sox2/Oct3-4 heterodimer transcription factor binding domain (bd) followed by a 165-bp minimal FGF4 promoter (mFGF4). This plasmid was a gift of Claudio Basilico (New York University). To increase the FP signal, the Sox2/Oct 3-4-mFGF regulatory fragment was moved to the “act-GVP-UG” concatenated plasmid created by Köster and Fraser (2001), which was adapted from the Drosophila Gal4-UAS system (Brand and Perrimon,1993) and shown to function in Xenopus (Chae et al.,2002; Hartley et al.,2002). We replaced the minimal promoters of the act-GVP-UG plasmid with our Sox2/Oct3-mFGF regulatory fragment to control the expression of the transcriptional activator, Gal4-VP16, which in turn drives the expression of genes controlled by the upstream activating sequence (UAS).
The final expression vector (abbreviated as pSox2-bd::FP) contains: 6 repeats of the Sox2/Oct3-4 transcription factor binding domain, mFGF4, Gal4-VP16, a polyadenylation site, 14 repeats of UAS, mFGF4, and eGFP (ClonTech, Palo Alto, CA).
Sequencing with EGFP-N identified 1nt deletion at position 1733, 1 nt insertion at position 1214, 2 nt insertion at 1557 compared to the depositor provided sequence. These mutations are all in non-coding regions and have been shown by the depositing lab to not effect the function of the plasmid.
Proper citation: RRID:Addgene_34703 Copy
Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110587
Comments: 3 amino acids, GSM, were introduced after aa148 for cloning purposes.
Proper citation: RRID:Addgene_34702 Copy
Species: Homo sapiens
Genetic Insert: phytanoyl-CoA hydroxylase interacting protein-like
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34823 Copy
Species: Homo sapiens
Genetic Insert: NRF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7000; Vector Backbone:pMSCV-hyg; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20385086
Proper citation: RRID:Addgene_34707 Copy
Species: Homo sapiens
Genetic Insert: FLJ30046
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34828 Copy
Species: Homo sapiens
Genetic Insert: C10orf83
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34827 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B0;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34651 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B0;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34657 Copy
Species: Homo sapiens
Genetic Insert: cytokine induced apoptosis inhibitor 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34811 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B2;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34656 Copy
Species: Homo sapiens
Genetic Insert: F-box protein 31
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34815 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B2;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34659 Copy
Species: Homo sapiens
Genetic Insert: C6orf60
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34813 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B1;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34658 Copy
Species: Homo sapiens
Genetic Insert: RAB17
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34812 Copy
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