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Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV066; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177703 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV066; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177704 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mCherry
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177706 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34614191
Proper citation: RRID:Addgene_177666 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV066; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177701 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34614191
Proper citation: RRID:Addgene_177667 Copy
Species: Homo sapiens
Genetic Insert: HSPA1L
Vector Backbone Description: Backbone Size:3352; Vector Backbone:pT7C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972319
Comments: Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
Proper citation: RRID:Addgene_177660 Copy
Species: Homo sapiens
Genetic Insert: HSP90AA1
Vector Backbone Description: Backbone Size:3352; Vector Backbone:pT7C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972319
Comments: Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
Proper citation: RRID:Addgene_177658 Copy
Species: Mus musculus
Genetic Insert: Myc
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7657; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28089889
Proper citation: RRID:Addgene_177650 Copy
Species: Homo sapiens
Genetic Insert: Tau (2N4R)
Vector Backbone Description: Backbone Size:3256; Vector Backbone:pT7II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972319
Comments: Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
Proper citation: RRID:Addgene_177653 Copy
Species: Homo sapiens
Genetic Insert: DNAJA1
Vector Backbone Description: Backbone Size:3352; Vector Backbone:pT7C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972319
Comments: Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
Proper citation: RRID:Addgene_177655 Copy
Species: Homo sapiens
Genetic Insert: DNAJB6
Vector Backbone Description: Backbone Size:3352; Vector Backbone:pT7C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972319
Comments: Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
Proper citation: RRID:Addgene_177657 Copy
Species: Moraxella bovoculi AAX08_00205
Genetic Insert: FokI-dMb2Cas12a
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible FokI-dMb2Cas12a entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35377968
Proper citation: RRID:Addgene_177684 Copy
Species: Moraxella bovoculi AAX08_00205
Genetic Insert: 2xFokI-dMb2Cas12a
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible 2xFokI-dMb2Cas12a entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35377968
Proper citation: RRID:Addgene_177686 Copy
Genetic Insert: FokI-dCas9-SpRY
Vector Backbone Description: Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible FokI-dCas9-SpRY entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35377968
Proper citation: RRID:Addgene_177682 Copy
Species: Other
Genetic Insert: hFluc
Vector Backbone Description: Backbone Marker:Addgene #177714; Vector Backbone:phFluc-WT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34857875
Proper citation: RRID:Addgene_177715 Copy
Species: Synthetic
Genetic Insert: DREADD Gi-mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4591; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, INTRSECT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177673 Copy
Species: Synthetic
Genetic Insert: DREADD Gi-mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4591; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, INTRSECT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177672 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177711 Copy
Species: Drosophila melanogaster
Genetic Insert: miR-1006 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1006-psm (gagctcCTATGAATAAGAAATCCATTATAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17771 Copy
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