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Species: Homo sapiens
Genetic Insert: PTTG1 (Guide Designation A9.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90862 Copy
Species: Homo sapiens
Genetic Insert: PTTG1 (Guide Designation G10.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90861 Copy
Species: Homo sapiens
Genetic Insert: PTTG1 (Guide Designation A10.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90863 Copy
Species: Homo sapiens
Genetic Insert: PTTG1 (Guide Designation G9.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90860 Copy
Species: Homo sapiens
Genetic Insert: UNG (Guide Designation D11.3)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90936 Copy
Species: Homo sapiens
Genetic Insert: UBE2C (Guide Designation H6.2)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90935 Copy
Species: Homo sapiens
Genetic Insert: VCP (Guide Designation F11.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90938 Copy
Species: Homo sapiens
Genetic Insert: UBA1 (Guide Designation G5.3)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90932 Copy
Species: Homo sapiens
Genetic Insert: SMC6 (Guide Designation G3.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90898 Copy
Species: Homo sapiens
Genetic Insert: UBA1 (Guide Designation G6.3)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90933 Copy
Species: Homo sapiens
Genetic Insert: SMC3 (Guide Designation H8.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90895 Copy
Species: Homo sapiens
Genetic Insert: SMC3 (Guide Designation H7.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90894 Copy
Species: Homo sapiens
Genetic Insert: TUBG1 (Guide Designation F7.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90930 Copy
Species: Homo sapiens
Genetic Insert: TUBE1 (Guide Designation F6.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90929 Copy
Species: Homo sapiens
Genetic Insert: TUBB (Guide Designation F1.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90924 Copy
Species: Homo sapiens
Genetic Insert: TUBD1 (Guide Designation F4.4)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90927 Copy
Species: Homo sapiens
Genetic Insert: SKA1 (Guide Designation H3.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90888 Copy
Species: Homo sapiens
Genetic Insert: TRIP13 (Guide Designation H4.2)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90921 Copy
Species: Homo sapiens
Genetic Insert: SGO1 (Guide Designation H2.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90887 Copy
Species: Homo sapiens
Genetic Insert: TTK (Guide Designation H10.1)
Vector Backbone Description: Backbone Marker:Eric Lander & David Sabatini labs (Addgene plasmid #71409); Backbone Size:9151; Vector Backbone:pLenti-sgRNA; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Comments: Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
Proper citation: RRID:Addgene_90923 Copy
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