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Species: Homo sapiens
Genetic Insert: E2F-1
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1638635
Proper citation: RRID:Addgene_21668 Copy
Species: Homo sapiens
Genetic Insert: Orai1
Vector Backbone Description: Backbone Size:7480; Vector Backbone:MO70; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17293345
Comments: See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).
Proper citation: RRID:Addgene_21662 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Constructs for Soft-agar assay
Proper citation: RRID:Addgene_21661 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation
Proper citation: RRID:Addgene_21639 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4969; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1638635
Proper citation: RRID:Addgene_21750 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722
Proper citation: RRID:Addgene_21754 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722
Proper citation: RRID:Addgene_21753 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722
Proper citation: RRID:Addgene_21752 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Proper citation: RRID:Addgene_21645 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1 UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Proper citation: RRID:Addgene_21640 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 short UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Proper citation: RRID:Addgene_21644 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Proper citation: RRID:Addgene_21643 Copy
Species: Homo sapiens
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19587239
Proper citation: RRID:Addgene_21733 Copy
Species: Homo sapiens
Genetic Insert: hDlg/SAP97 PDZ2 domain
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17713926
Comments: There is a second His tag that is C-terminal on the vector after the first His tag.It is however not in frame with the insert and other His tag.
Proper citation: RRID:Addgene_21692 Copy
Species: Homo sapiens
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19587239
Proper citation: RRID:Addgene_21732 Copy
Species: Homo sapiens
Genetic Insert: Histone Deacetylase 5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19131628
Comments: Wild type HDAC5 protein with degenerate codons
Proper citation: RRID:Addgene_21626 Copy
Species: Homo sapiens
Genetic Insert: hOCT4 - 2A - hSOX2 - 2A - hKLF4
Vector Backbone Description: Backbone Size:9692; Vector Backbone:pDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19415770
Comments: Plasmid constructed by Kevin Pawlik
Proper citation: RRID:Addgene_21627 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4969; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1638635
Proper citation: RRID:Addgene_21748 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Proper citation: RRID:Addgene_32971 Copy
Species: Homo sapiens
Genetic Insert: CYCLIN F
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7791; Vector Backbone:pLVX-Tight-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Backbone vector, pLVX-Tight-Puro map is attached.
Proper citation: RRID:Addgene_32977 Copy
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