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Species: Synthetic
Genetic Insert: FACS Controls
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: Please note the fusion site D (AGGT) appears twice between the GFP CDS and the terminator. This duplication has no affect on plasmid function.
Proper citation: RRID:Addgene_66064 Copy
Species: Synthetic
Genetic Insert: FACS Controls
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Proper citation: RRID:Addgene_66065 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66101 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66069 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chronos
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66102 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66067 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:2455; Vector Backbone:pAH63; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25849635
Proper citation: RRID:Addgene_66073 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66071 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479688
Proper citation: RRID:Addgene_66072 Copy
Species: Homo sapiens
Genetic Insert: human Vimentin - APEX2
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3970; Vector Backbone:pECFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25419960
Proper citation: RRID:Addgene_66170 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029110. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66188 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029113 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66190 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029112 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66191 Copy
Species: Homo sapiens
Genetic Insert: XRN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4174; Vector Backbone:pLNHA-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23142987
Proper citation: RRID:Addgene_66596 Copy
Species: Homo sapiens
Genetic Insert: Sec16B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22355596
Proper citation: RRID:Addgene_66607 Copy
Species: Homo sapiens
Genetic Insert: COL1A1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11884515
Comments: Insert contains T1434S compared to all available reference sequences. Depositor states that this discrepancy does not affect plasmid function.
Proper citation: RRID:Addgene_66602 Copy
Species: Homo sapiens
Genetic Insert: TPST2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18522538
Comments: Molecular cloning of TPST (tyrosylprotein sulfotransferase)–EGFP (enhanced green fluorescent protein)
The TPST1 and TPST2 sequences were obtained from human ESTs (expressed sequence tags) from the RZPD (Deutsches Ressourcenzentrum für Genomforschung, Berlin, Germany; GenBank® accession numbers NM_003596 and AF061254 respectively). TPST1–EGFP and TPST2–EGFP were obtained by PCR using the following primers: plus strand, 5′-GGGGCTCGAGATGGTTGGAAAGCTGAAG-3′, and minus strand, 5′-GGGGCTGCAGCTCCACTTGCTCAGTC-3′ for TPST1; and plus strand, 5′-GGGGCTCGAGATGCGCCTGTCGGTGC-3′, and minus strand, 5′-GGGGCTGCAGCGAGCTTCCTAAGTGG-3′ for TPST2 (XhoI and PstI, restriction sites are underlined). PCR products were purified and ligated into the pGEM-T-Easy vector (Promega) following the manufacturer's instructions. Positive clones were digested with XhoI and PstI, and the fragments obtained were inserted in the corresponding sites of pEGFP-N1 (Clontech). All constructs were verified by double-strand DNA sequencing (MWG Biotech, Ebersburg, Germany). Two differences to the published sequence of TPST2 were identified (276C>T and 1002T>C, relative to the start codon), but neither of which affect the predicted protein sequence.
Insert contains T370A relative to reference sequence. The depositing laboratory does not know the effect of this substitution on gene function
Proper citation: RRID:Addgene_66618 Copy
Species: Mus musculus
Genetic Insert: Sec23B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22355596
Comments: Depositor states that mutations do not not affect function.
Proper citation: RRID:Addgene_66615 Copy
Species: Mus musculus
Genetic Insert: Sec23B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22355596
Proper citation: RRID:Addgene_66616 Copy
Species: Homo sapiens
Genetic Insert: Sec24D
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10825291
Proper citation: RRID:Addgene_66614 Copy
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