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Species: Synthetic
Genetic Insert: HyperdCas12a
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35414015
Proper citation: RRID:Addgene_183964 Copy
Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183071 Copy
Species: Synthetic
Genetic Insert: Cas12c tracrRNA and pre-crRNA
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Comments: A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
Proper citation: RRID:Addgene_183076 Copy
Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:Modified pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659325
Comments: This Cas12c bacterial expression plasmid was originally created by Harrington et al., 2020 and now modified (R965H) to match with the wild-type protein sequence of Cas12c_4.
Proper citation: RRID:Addgene_183069 Copy
Species: Synthetic
Genetic Insert: huAsCpf1
Vector Backbone Description: Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918548
Comments: Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
Proper citation: RRID:Addgene_84739 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:9657; Vector Backbone:Addgene #136936; Vector Types:Mammalian Expression, CRISPR, TALEN, Synthetic Biology, Donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35608547
Comments: Please note that this plasmid is identical to Addgene plasmid # 136936 except it does not contain the WPRE sequence.
Proper citation: RRID:Addgene_179837 Copy
Species: Synthetic
Genetic Insert: EGFP-TM
Vector Backbone Description: Backbone Size:5236; Vector Backbone:pPyPGK-IRES-Pac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35616331
Proper citation: RRID:Addgene_183606 Copy
Species: Synthetic
Genetic Insert: actin > GenR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:1835; Vector Backbone:pUC57-mini; Vector Types:Capsaspora owczarzaki expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659869
Proper citation: RRID:Addgene_176479 Copy
Species: Synthetic
Genetic Insert: Lifeact peptide
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pJP103; Vector Types:Capsaspora owczarzaki expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659869
Proper citation: RRID:Addgene_176494 Copy
Species: Synthetic
Genetic Insert: actin > NatR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:1835; Vector Backbone:pUC57-mini; Vector Types:Capsaspora owczarzaki expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659869
Proper citation: RRID:Addgene_176480 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34477863
Comments: Plasmid parts were synthesized or taken from the Yeast MoClo kit.
Proper citation: RRID:Addgene_183057 Copy
Species: Synthetic
Genetic Insert: LaG17 GFP nanobody SynNotch tTA
Vector Backbone Description: Backbone Size:3307; Vector Backbone:attB53-Pac-attB53 (Addgene 183610); Vector Types:Mammalian Expression, RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35616331
Proper citation: RRID:Addgene_183614 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34477863
Comments: Plasmid parts were synthesized or taken from the Yeast MoClo kit.
Proper citation: RRID:Addgene_183060 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183222 Copy
Species: Synthetic
Genetic Insert: Synthetic construct iC++ gene
Vector Backbone Description: Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98165 Copy
Species: Synthetic
Genetic Insert: Synthetic construct Aurora gene
Vector Backbone Description: Backbone Size:4714; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98167 Copy
Species: Synthetic
Genetic Insert: Synthetic construct Phobos gene
Vector Backbone Description: Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98166 Copy
Species: Synthetic
Genetic Insert: Synthetic construct iC++_C128A gene
Vector Backbone Description: Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98168 Copy
Species: Synthetic
Genetic Insert: Synthetic construct Aurora_C128A gene
Vector Backbone Description: Backbone Size:4714; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98170 Copy
Species: Synthetic
Genetic Insert: Synthetic construct iChR2++ gene
Vector Backbone Description: Backbone Size:4714; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29097684
Proper citation: RRID:Addgene_98172 Copy
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