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Species: M. smegmatis
Genetic Insert: MSMEG_5471
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167434 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5472
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167435 Copy
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3).
1. First development of pSelAct for knockouts in Rhodococcus equi:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans:
Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022
3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174374 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34083482
Proper citation: RRID:Addgene_174517 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0948
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174952 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0952
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174954 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0953
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174955 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0954
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174956 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0956
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174957 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0970
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174959 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0946
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174950 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0943
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174949 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0933
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174941 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0934
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174942 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0937
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174945 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0938
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174946 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0939
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174947 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0929
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174940 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0977
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174964 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0971
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_174960 Copy
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