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Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mKO; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 548; Emission = 561
Proper citation: RRID:Addgene_57847 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mKO2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18454154
Comments: R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 551; Emission = 565
Proper citation: RRID:Addgene_57867 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mKOet; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 548; Emission = 561
Proper citation: RRID:Addgene_57930 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mOrange2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18454154
Comments: R. norvegicus (rat) Beta-2 Connexin 26. Excitation = 549; Emission = 565
Proper citation: RRID:Addgene_57949 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mOrange2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18454154
Comments: R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 549; Emission = 565
Proper citation: RRID:Addgene_57951 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTurboRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 553; Emission = 576
Proper citation: RRID:Addgene_58052 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58109 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTurboRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 553; Emission = 576
Proper citation: RRID:Addgene_58061 Copy
Species: Rattus norvegicus
Genetic Insert: CEBPA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58080 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn2beta AS4(-)
Vector Backbone Description: Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I).
Insert can also be released by single digest using XhoI.
The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58268 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58267 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58266 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: Please see Addgene plasmid 40755 for more information on GCaMP6f; also note that GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58514 Copy
Species: Rattus norvegicus
Genetic Insert: alpha2 delta-1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22678293
Proper citation: RRID:Addgene_58730 Copy
Species: Rattus norvegicus
Genetic Insert: S6K
Vector Backbone Description: Vector Backbone:pHR-IG (pHR’tripCMV-IRES-eGFP); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25013017
Comments: The S6K1 gene was cloned from Addgene plasmid # 26610, pcDNA-Myc S6K1. It starts at aa24 compared to GenBank reference sequence NP_114191.1 and contains an additional 7 amino acids after the end of S6K1 gene in this reference sequence and plasmid 26610.
Proper citation: RRID:Addgene_59446 Copy
Species: Rattus norvegicus
Genetic Insert: Gata2
Vector Backbone Description: Backbone Marker:Thomson lab; Backbone Size:4179; Vector Backbone:pBTO-Dest; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25458896
Proper citation: RRID:Addgene_60665 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23363606
Proper citation: RRID:Addgene_60702 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23363606
Proper citation: RRID:Addgene_60701 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23363606
Proper citation: RRID:Addgene_60700 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23363606
Proper citation: RRID:Addgene_60706 Copy
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