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Species: Homo sapiens
Genetic Insert: gp78c
Vector Backbone Description: Backbone Marker:Amersham/GE; Backbone Size:5000; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11724934
Proper citation: RRID:Addgene_11429 Copy
Species: Homo sapiens
Genetic Insert: LMNB1 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114403 Copy
Species: Homo sapiens
Genetic Insert: SMC1A Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SMC1A in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: LSSSGPSGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114406 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4537; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114472 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5603; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114471 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5372; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114469 Copy
Species: S. pyogenes, E. coli
Genetic Insert: SpCas9, Ec86-RT
Vector Backbone Description: Vector Backbone:pRS403; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30245013
Proper citation: RRID:Addgene_114454 Copy
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR, CEN/ARS; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30245013
Proper citation: RRID:Addgene_114451 Copy
Species: Homo sapiens
Genetic Insert: PPAR gamma 2
Vector Backbone Description: Backbone Marker:pBabe-zeo is available at Addgene; Backbone Size:5169; Vector Backbone:pBabe-zeo (aka: pBabe-bleo); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Human PPARg2 (-10 to 1515 TAG) - insert was cut with XhoI and cloned into vector cut with SalI.
Proper citation: RRID:Addgene_11439 Copy
Species: Homo sapiens
Genetic Insert: RXR alpha
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11441 Copy
Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114443 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114442 Copy
Species: Homo sapiens
Genetic Insert: PYL-HDAC3
Vector Backbone Description: Backbone Size:5416; Vector Backbone:pAAV-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34494094
Proper citation: RRID:Addgene_114394 Copy
Genetic Insert: ChrimsonR-tdTomato, tTA2
Vector Backbone Description: Backbone Size:15017; Vector Backbone:TIGRE2.0 targeting vector; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30007418
Proper citation: RRID:Addgene_114431 Copy
Species: Botrytis cinerea
Genetic Insert: BcLOV4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:BamUK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30065115
Proper citation: RRID:Addgene_114596 Copy
Species: Mus musculus
Genetic Insert: anti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750815. Isotype: IgG2a. Additional species: human, mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114544 Copy
Species: Mus musculus
Genetic Insert: mE6-AP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9182527
Proper citation: RRID:Addgene_11468 Copy
Genetic Insert: SNAP
Vector Backbone Description: Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Proper citation: RRID:Addgene_114696 Copy
Genetic Insert: NuMA(1-2115ΔNLS)-RFP-Nano
Vector Backbone Description: Vector Backbone:pMK247_addgene#105926; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29848445
Comments: Please note that some discrepancies were found between Addgene's quality control result and the depositor's full plasmid sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_114699 Copy
Species: Medaka fish (Oryzias latipes)
Genetic Insert: Tol2 transposase
Vector Backbone Description: Backbone Marker:Koichi Kawakami; Vector Backbone:pCAGGS-T2TP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504719
Proper citation: RRID:Addgene_114725 Copy
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