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Genetic Insert: Roxed-Cre
Vector Backbone Description: Vector Backbone:pTS(2); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51276 Copy
Species: Bacillus megaterium
Genetic Insert: BM1P1
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51157 Copy
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51278 Copy
Species: Escherichia coli
Genetic Insert: BetI
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51156 Copy
Genetic Insert: NLS-HA-Dre-P2A-BFP
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51277 Copy
Genetic Insert: Dre
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Comments: Please note that the complementary plasmid pCAG-Roxed-Cre (www.addgene.org/51273) must be ordered with pCAG-NLS-HA-Dre in order to successfully use the Co-Driver recombinase system as described in the associated publication.
Proper citation: RRID:Addgene_51272 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: AmrR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51150 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1
Vector Backbone Description: Backbone Marker:R.Y. Tsien, PNAS 2002 v99 pp7877–7882; Vector Backbone:pmRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24391794
Comments: This cofilin plasmid contains some silent mutations to make it resistant to an siRNA used in the Bamburg lab. The mutations change the wildtype sequence (AAG TCT TCA ACG CCA GAG GAG) into AAG AGC TCA ACC CCA GAG GAG.
Proper citation: RRID:Addgene_51279 Copy
Genetic Insert: E6LLO
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23832121
Proper citation: RRID:Addgene_51281 Copy
Species: Bacillus megaterium
Genetic Insert: Cyp106
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51166 Copy
Species: Homo sapiens
Genetic Insert: U6
Vector Backbone Description: Backbone Marker:Addgene plasmid 51283; Vector Backbone:pAluYa5-neo-TET; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390602
Comments: U6 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.
Proper citation: RRID:Addgene_51286 Copy
Species: Enterococcus faecalis
Genetic Insert: Ef0113
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51168 Copy
Species: Homo sapiens
Genetic Insert: L1 ORF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4595; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:21572950
Comments: This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence.
Proper citation: RRID:Addgene_51289 Copy
Species: Homo sapiens
Genetic Insert: L1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572950
Comments: L1 is tagged with neoI indicator cassette containing an inverted neomycin resistance gene. The SV40 promoter (SV40p) drives transcription of the neomycin gene which is disrupted by an intron in the opposite orientation that renders it non-functional. The intron is only spliced out from tagged L1 transcripts generated by the CMV promoter. When retrotransposition of the spliced L1 RNA occurs, the new insert will contain a functional neo gene that is expressed from the SV40 promoter.
The L1 open reading frames were codon optimized using Primo Optimum 3.4 (http://www.changbioscience.com/primo/primoo.html), which makes synonymous changes to optimal codons within the nucleotide sequence. A 63 bp inter-ORF with 92% sequence identity to L1RP was utilized for all constructs. Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction. The optimized nucleotide sequence is 74.9% identical to the sequence of L1RP.
Proper citation: RRID:Addgene_51288 Copy
Species: Rhodococcus erythropolis
Genetic Insert: CampR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51162 Copy
Species: Micromonospora echinospora
Genetic Insert: CalR1
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51161 Copy
Species: E. coli
Genetic Insert: E. coli RtcB
Vector Backbone Description: Backbone Marker:EMD / Novagen; Backbone Size:5230; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26001965
Comments: Use expression strain: BL21 (DE3) RIPL
Proper citation: RRID:Addgene_51282 Copy
Species: Rhizobium etli
Genetic Insert: CasR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51163 Copy
Species: Homo sapiens
Genetic Insert: L1RE1
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390602
Comments: L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Proper citation: RRID:Addgene_51284 Copy
Species: Mycobacterium tubercolosis
Genetic Insert: EthR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51170 Copy
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