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Species: Synthetic
Genetic Insert: human codon optimized AcrVA2 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115138 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA3.1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115139 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKR
Proper citation: RRID:Addgene_115120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1151 Copy
Species: Mus musculus
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28842570
Proper citation: RRID:Addgene_115341 Copy
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8724; Vector Backbone:pDest-590; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11519 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8266; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11518 Copy
Species: Mus musculus
Genetic Insert: shRNA targeting Plxnb1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29981480
Proper citation: RRID:Addgene_115174 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8932; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11520 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1153 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLX317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115439 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erg11-iGFPx3
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Proper citation: RRID:Addgene_115422 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: vps10-104
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Please note sequence is from JK93da yeast strain rather than S288C
Proper citation: RRID:Addgene_115426 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pTK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 122. BoxB repeat sequence: gggccctgaagaagggccc
Proper citation: RRID:Addgene_115365 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16081698
Comments: clone number 137
Proper citation: RRID:Addgene_115366 Copy
Genetic Insert: HA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 211
Proper citation: RRID:Addgene_115360 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1154 Copy
Species: bacteriophage P1
Genetic Insert: Phosphoglycerate kinase 1 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2906; Vector Backbone:XhopKS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with the NLS-Cre.
NOTE: There are some discrepancies between the Addgene quality control sequence and the sequence provided by the depositing lab, but these differences should not affect plasmid function.
Proper citation: RRID:Addgene_11543 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein
Vector Backbone Description: Vector Backbone:pRS406-ADH1/CYC1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30804216
Comments: Please visit https://www.biorxiv.org/content/10.1101/345637v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_115565 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10797013
Proper citation: RRID:Addgene_11558 Copy
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