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Species: Other
Genetic Insert: BIN2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AVM/
Proper citation: RRID:Addgene_53657 Copy
Species: Other
Genetic Insert: CDC42BPAA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His, TEV-cleavable, for LIC cloning. EXP-10-AE0544 http://www.thesgc.org/structures/4AW2/
Proper citation: RRID:Addgene_53658 Copy
Species: Other
Genetic Insert: ETV1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AVP/
Proper citation: RRID:Addgene_53655 Copy
Species: Other
Genetic Insert: SNX3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2YPS/
Proper citation: RRID:Addgene_53665 Copy
Species: Other
Genetic Insert: SNX27A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4HAS/
Proper citation: RRID:Addgene_53662 Copy
Species: Other
Genetic Insert: TSTA3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4B8Z/
Proper citation: RRID:Addgene_53663 Copy
Species: Other
Genetic Insert: NEK1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4APC/
Proper citation: RRID:Addgene_53661 Copy
Species: Other
Genetic Insert: SNX14B
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BGJ/
Proper citation: RRID:Addgene_53668 Copy
Species: Other
Genetic Insert: SOCS4B
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2IZV/
Proper citation: RRID:Addgene_53669 Copy
Species: Other
Genetic Insert: FEVA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2YPR/
Proper citation: RRID:Addgene_53666 Copy
Species: Other
Genetic Insert: MYO5BA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4J5M/
Proper citation: RRID:Addgene_53667 Copy
Species: Other
Genetic Insert: CDKL5A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/4BGQ/
Proper citation: RRID:Addgene_53686 Copy
Species: Other
Genetic Insert: SOCS2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pLIC-SGC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: T7-lacl regulated, N-terminal his tag, TEV cleavage, LIC cloning using BseRI/ T4 pol, pET backbone. http://www.thesgc.org/structures/2C9W/ (complex)
Proper citation: RRID:Addgene_53689 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Other
Genetic Insert: 2x membrane linker
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19378112
Comments: Use NotI/SP6 for RNA to inject.
Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean.
This puts membrane-cerulean between XbaI and SnaBI of pCS.
Proper citation: RRID:Addgene_53749 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Ras GTPase
Proper citation: RRID:Addgene_54243 Copy
Species: Other
Genetic Insert: Peroximal Targeting Signal 1 (PTS1)
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mEmerald-Peroxisomes; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Peroximal Targeting Signal 1 (PTS1). Excitation = 487; Emission = 509. Localization data: Peroxisomes
Proper citation: RRID:Addgene_54228 Copy
Species: Other
Genetic Insert: mCherry and EGFP
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBS SK(+); Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28421278
Proper citation: RRID:Addgene_54322 Copy
Species: Other
Genetic Insert: cauliflower mosaic virus 35S promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54409 Copy
Species: Other
Genetic Insert: L. japonicus polyubiquitin promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54408 Copy
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