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Species: Saccharomyces cerevisiae
Genetic Insert: Ubi-M
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65148 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubi-R
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65150 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tSSA1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65159 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tADH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65162 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 5' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Combination of part Types 7, 8a, and 8b are used for Yeast Chromosomal Integration in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65172 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tPGK1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65171 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tTDH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
This plasmid stock has been found to contain dimers. Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65173 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65183 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65182 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65181 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CEN6/ARS4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 3' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65194 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 3' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65193 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CYC1 promoter
Vector Backbone Description: Vector Backbone:HCKan-P; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65338 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF2 promoter
Vector Backbone Description: Vector Backbone:HCKan-P; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 terminator
Vector Backbone Description: Vector Backbone:HCKan-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65339 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pMV; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65334 Copy
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