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Species: Catharanthus roseus
Genetic Insert: Catharanthus roseus STR1 promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus. Can be used as a control for transactivation experiments. Confirmed activation through ORCA3 and repression through ZCT1
Proper citation: RRID:Addgene_123186 Copy
Species: Catharanthus roseus
Genetic Insert: ORCA3 from Catharanthus roseus
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Octadecanoid-Responsive Catharanthus AP2 (ORCA3, GenBank: EU072424.1); activator of the Catharanthus roseus STR1 promoter
Proper citation: RRID:Addgene_123185 Copy
Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113637 Copy
Genetic Insert: Synpcc7942_1849
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30540929
Proper citation: RRID:Addgene_119795 Copy
Genetic Insert: Synpcc7942_1557
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30540929
Proper citation: RRID:Addgene_119796 Copy
Species: Broad range of genetically tractable cyanobacteria
Genetic Insert: RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
Vector Backbone Description: Vector Backbone:RSF1010Y25F-AADA; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:25149516
Proper citation: RRID:Addgene_120085 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
Vector Backbone Description: Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:27902432
Proper citation: RRID:Addgene_120087 Copy
Species: Other
Genetic Insert: Transposon Tn5-692
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:12218043
Comments: Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham.
NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest.
Additional references:
Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143
Proper citation: RRID:Addgene_70284 Copy
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:17639350
Comments: MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6)
Proper citation: RRID:Addgene_70692 Copy
Species: Arabidopsis thaliana
Genetic Insert: Telomere
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:17085598
Comments: Please note that Addgene NGS found a 1.5kb deletion in the telomere repeats as compared to the original depositor sequence. The depositor has confirmed that the telomere sequence in the plasmid is inherently unstable, and that the plasmid is still functional for telomere truncation.
The plasmid was also verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes.
Proper citation: RRID:Addgene_65721 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160171 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160169 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160261 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160262 Copy
Species: Other
Genetic Insert: aadA
Vector Backbone Description: Backbone Marker:-; Backbone Size:7000; Vector Backbone:pAM5406; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31585408
Proper citation: RRID:Addgene_132663 Copy
Species: C. tetani
Genetic Insert: Tetanus toxin light chain domain
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128402 Copy
Species: P. aeruginosa
Genetic Insert: Exotoxin A
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128404 Copy
Species: H. influenzae
Genetic Insert: H. influenzae protein D
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128399 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100917 Copy
Vector Backbone Description: Vector Backbone:pDFC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:41699564
Proper citation: RRID:Addgene_251363 Copy
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