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Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258197 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258199 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258190 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258193 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258191 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258192 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sc Top2
Vector Backbone Description: Vector Backbone:pRS402/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_257905 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sc Top1
Vector Backbone Description: Vector Backbone:pRS404/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_257904 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sc Pol31
Vector Backbone Description: Vector Backbone:pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_257902 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258201 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258202 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sc RFC5 (mutant)
Vector Backbone Description: Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258210 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258206 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258205 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC1
Vector Backbone Description: Vector Backbone:pLANT(3b)/RIL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mix & Match (co-transform) with different versions of Sc RFC[2+3+4], either wild-type or containing various mutations.
Proper citation: RRID:Addgene_258209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps10-FRBx2-HA-msGFPx3
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255062 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps52-FRBx2-HA
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255065 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps52-msGFP2x2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255067 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YKU70 sgRNA
Vector Backbone Description: Backbone Marker:John Wyrick lab; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:42519825
Comments: This plasmid contains an example guide sequence targeting **YKU70**. As described in our protocol paper, the guide sequence can be easily changed by designing forward and reverse primers for a new target and then using PCR and seamless plasmid recircularization.
Proper citation: RRID:Addgene_254712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PtGFP
Vector Backbone Description: Backbone Marker:Andrew Gleave (Addgene #247985); Backbone Size:5042; Vector Backbone:pART7; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16600023
Comments: pART7::COX:PtGFP is for targeting PtGFP to the plant mitochondria matrix. The PtGFP gene is concatenated downstream of a sequence coding for the signal sequence (first 29 aminoacids) of the cytochrome oxidase subunit IV from yeast (COX-IV; Gene Bank X01418; Maarse et al. 1984, doi.org/10.1002/j.1460-2075.1984.tb02216.x; Akashi et al. 1998, doi.org/10.1016/S0014-5793(98)00717-0; Filipin et al. 2005, doi.org/10.1016/j.ceca.2004.08.002; Llopis et al. 1998, doi.org/10.1073/pnas.95.12.6803 ). A 5'UTR 144 nucleotide translation leader sequence from the tobacco etch virus (TEV; Gene Bank GU734657) was prepended for enhancement of expression (Carrington and Freed 1990 doi.org/10.1128/jvi.64.4.1590-1597.1990; Zeenko and Gallie 2005, DOI: 10.1074/jbc.M503576200; Peeters et al. 2000, doi.org/10.1007/s002390010044; Harper et al. 1998, doi.org/10.1074/jbc.273.2.1099). PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695).
Proper citation: RRID:Addgene_258581 Copy
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