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Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37646 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37647 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37642 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37643 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37644 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37641 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37834 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1W32A;Y35A::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37835 Copy
Species: Caenorhabditis elegans
Genetic Insert: F13D12.6(G166R)
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37837 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ub-V
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37839 Copy
Species: Caenorhabditis elegans
Genetic Insert: cbr-unc-119::FRT*::galk::FRT*
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression, recombineering; Bacterial Resistance:Ampicillin
Comments: This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene.
The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.
Proper citation: RRID:Addgene_38149 Copy
Species: Caenorhabditis elegans
Genetic Insert: ptc-3::GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21215260
Comments: The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5′ upstream sequence, including the putative promoter
Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_38145 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-60p::ptc-3::gfp
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21215260
Comments: The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)
Proper citation: RRID:Addgene_38146 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3
Vector Backbone Description: Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171208 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171207 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171205 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171204 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171203 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28811311
Proper citation: RRID:Addgene_107020 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-PH
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28811311
Proper citation: RRID:Addgene_107016 Copy
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