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Species: Synthetic
Genetic Insert: Nano-lantern_CaM-2GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859494
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51985 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859502. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51991 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859502. Although they differ by only three N-terminal amino acids in the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51992 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2870; Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To clone new genes for your proteins of interest into the pCRT7 NT vector provided, use 5’KpnI--‐3’HindIII restriction sites.
See Lutz and Bujard 1997 (PMID 9092630) for information on the tetR/pLtetO promoter system used for inducible expression in the pCRT7 NT vector.
The phosphoproteins (or sfGFP) expressed via the pCRT7 pLtetO/tetR plasmid is induced with 100 ng/mL anhydrotetracycline.
Proper citation: RRID:Addgene_52053 Copy
Species: Synthetic
Genetic Insert: GCaMP6s-CAAX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4005; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24463606
Comments: GCaMP6s (Addgene plasmid 40753) tagged with CAAX motif (amino acids KEKMSKDGKKKKKKSKTKCVIM)
Proper citation: RRID:Addgene_52228 Copy
Species: Synthetic
Genetic Insert: PRHIII-sfGFP
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24788326
Comments: Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.
Proper citation: RRID:Addgene_52217 Copy
Species: Synthetic
Genetic Insert: hrGFP
Vector Backbone Description: Backbone Size:8085; Vector Backbone:AAVS1-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24497442
Comments: Please note that this plasmid is NOT the inducible AAVS1-TRE3G-EGFP construct described in the associated publication.
Proper citation: RRID:Addgene_52344 Copy
Species: Synthetic
Genetic Insert: AAVS1 TALEN Right
Vector Backbone Description: Backbone Marker:Keith Joung lab(Addgene Plasmid # 32290) ; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24497442
Comments: Addgene's sequencing results identified a partial duplication within the TALEN repeat region of this plasmid relative to the expected sequence. The depositing lab confirmed that the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_52342 Copy
Species: Synthetic
Genetic Insert: AAVS1 TALEN Left
Vector Backbone Description: Backbone Marker:Keith Joung lab (Addgene Plasmid # 32290) ; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24497442
Comments: Addgene's sequencing results identified a partial duplication within the TALEN repeat region of this plasmid relative to the expected sequence. The depositing lab confirmed that the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_52341 Copy
Species: Synthetic
Genetic Insert: HD NG NN NN NN HD NG HD NG HD HD HD NI NG
Vector Backbone Description: Vector Backbone:pTal4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52412 Copy
Species: Synthetic
Genetic Insert: HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
Vector Backbone Description: Vector Backbone:pTal4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52413 Copy
Species: Synthetic
Genetic Insert: GFP-2A-puro
Vector Backbone Description: Backbone Size:2278; Vector Backbone:minimal Amp vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Proper citation: RRID:Addgene_52380 Copy
Species: Synthetic
Genetic Insert: Fusion NLS-EGFP-P2A-Cherry-CAAX
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5508; Vector Backbone:pcDNA3.1-(-)-Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52421 Copy
Species: Synthetic
Genetic Insert: GFP pA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Comments: This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881. We have introduced neo selection cassette at the 3' end of the cloned Oct4 locus.
Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52382 Copy
Species: Synthetic
Genetic Insert: fluorescent protein mpapaya
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094838
Proper citation: RRID:Addgene_52304 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin 2
Vector Backbone Description: Vector Backbone:pET-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24691867
Proper citation: RRID:Addgene_52319 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin 2
Vector Backbone Description: Vector Backbone:pET-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24691867
Proper citation: RRID:Addgene_52320 Copy
Species: Synthetic
Genetic Insert: ChopV
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13784; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22492054
Proper citation: RRID:Addgene_52476 Copy
Species: Synthetic
Genetic Insert: splitTVA-P2A-EGFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4436; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336151
Comments: Alternative plasmid name: pAAV-synP-FLEX-sTpE
Proper citation: RRID:Addgene_52474 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13896; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23945836
Comments: Alternative plasmid name: pRVdG-1E5SR
Proper citation: RRID:Addgene_52479 Copy
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