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  • Bacterial Resistance:ampicillin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET21a-Ef0113
 
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RRID:Addgene_51168 Ef0113 Enterococcus faecalis Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pBS-L1PA1-CH-mneo
 
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1+ mentions
RRID:Addgene_51288 L1 Homo sapiens Ampicillin PMID:21572950 L1 is tagged with neoI indicator cassette containing an inverted neomycin resistance gene. The SV40 promoter (SV40p) drives transcription of the neomycin gene which is disrupted by an intron in the opposite orientation that renders it non-functional. The intron is only spliced out from tagged L1 transcripts generated by the CMV promoter. When retrotransposition of the spliced L1 RNA occurs, the new insert will contain a functional neo gene that is expressed from the SV40 promoter. The L1 open reading frames were codon optimized using Primo Optimum 3.4 (http://www.changbioscience.com/primo/pri​moo.html), which makes synonymous changes to optimal codons within the nucleotide sequence. A 63 bp inter-ORF with 92% sequence identity to L1RP was utilized for all constructs. Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction. The optimized nucleotide sequence is 74.9% identical to the sequence of L1RP. Backbone Marker:Stratagene; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 4
pET21a-CampR
 
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RRID:Addgene_51162 CampR Rhodococcus erythropolis Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pET21a-CalR1
 
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1+ mentions
RRID:Addgene_51161 CalR1 Micromonospora echinospora Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 1
pET-53-RtcB
 
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RRID:Addgene_51282 E. coli RtcB E. coli Ampicillin PMID:26001965 Use expression strain: BL21 (DE3) RIPL Backbone Marker:EMD / Novagen; Backbone Size:5230; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 0
pET21a-CasR
 
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RRID:Addgene_51163 CasR Rhizobium etli Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:14 0
L1-neo-TET
 
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1+ mentions
RRID:Addgene_51284 L1RE1 Homo sapiens Ampicillin PMID:19390602 L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs. Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 6
pET21a-EthR
 
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RRID:Addgene_51170 EthR Mycobacterium tubercolosis Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pET21a-TylP
 
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RRID:Addgene_51210 TylP Streptomyces fradiae Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pcDNA3.1-mNRDc-E>A-V5
 
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RRID:Addgene_51298 NRDc E247A Mus musculus Ampicillin PMID:24492630 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E247A, enzymatic inactive mutant 2026-08-15 01:16:15 0
pcDNA3.1-mNRDc-V5
 
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RRID:Addgene_51297 NRDc Mus musculus Ampicillin PMID:24492630 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 0
pET21a-KstR
 
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RRID:Addgene_51179 KstR Rhodococcus erythropolis Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:14 0
pET21a-HemR
 
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RRID:Addgene_51173 HemR Propionibacterium freudenreichii Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pLenti.tie1.H2B.eYFP.W
 
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RRID:Addgene_51293 H2B-eYFP Homo sapiens Ampicillin PMID:20856866 Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2. Backbone Marker:Luigi Naldini; Backbone Size:6697; Vector Backbone:pRRLsin.cPPT.Tie1p.eGFP.wpre; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 0
pET-Ce25
 
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RRID:Addgene_51296 arginyl-tRNA synthetase Canavalia ensiformis (jack bean) Ampicillin PMID:18562210 For induction, use BL21CodonPlus, cool and grow at 23°C Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET32a+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin GTT (Val) insertion after initiation codon to permit NcoI cloning 2026-08-15 01:16:12 0
pET21a-HlyIIR
 
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RRID:Addgene_51174 HlyIIR Bacillus cereus Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pET21a-LitR
 
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RRID:Addgene_51181 LitR Vibrio fischeri Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
pET21a-MtrR
 
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RRID:Addgene_51188 MtrR Neisseria gonorrhoeae Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0
CMV-CC2
 
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RRID:Addgene_51221 DCTN1 Gallus gallus Ampicillin PMID:10525538 Backbone Size:4800; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa917-1040 2026-08-15 01:16:11 0
pET21a-NonG
 
Resource Report
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RRID:Addgene_51189 NonG Streptomyces griseus Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:11 0

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