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Vector Backbone Description: Vector Backbone: pLys (p15A origin of replication and CmR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109386 Copy
Genetic Insert: TEV protease
Vector Backbone Description: Vector Backbone:pBROBE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30247489
Proper citation: RRID:Addgene_110206 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Genetic Insert: E. coli K-12 MG1655_rLP5(nth-ydgR)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110245 Copy
Genetic Insert: E. coli K-12 MG1655_fLP2(yieN-trkD)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110242 Copy
Genetic Insert: E. coli K-12 MG1655_fLP3(ybbD-ylbG)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110243 Copy
Genetic Insert: E. coli K-12 MG1655_rLP6(ygcE-ygcF)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110246 Copy
Species: Synthetic
Genetic Insert: T7 promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110574 Copy
Species: Synthetic
Genetic Insert: CP25 Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110575 Copy
Species: Synthetic
Genetic Insert: CP32 Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110578 Copy
Species: Synthetic
Genetic Insert: CP12b Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110577 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Novagen/Agilent; Backbone Size:2412; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: pIM2432
Proper citation: RRID:Addgene_110547 Copy
Species: Synthetic
Genetic Insert: rpoC
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110592 Copy
Species: Synthetic
Genetic Insert: Lac promoter with lac operator and RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110582 Copy
Vector Backbone Description: Backbone Size:2979; Vector Backbone:pUCDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110742 Copy
Species: Synthetic
Genetic Insert: TetR and xCas9
Vector Backbone Description: Vector Backbone:p15A origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_111656 Copy
Vector Backbone Description: Backbone Size:2787; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29724956
Proper citation: RRID:Addgene_111986 Copy
Genetic Insert: Cas14b2
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112504 Copy
Genetic Insert: Cas14a1
Vector Backbone Description: Vector Backbone:pTet; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30337455
Proper citation: RRID:Addgene_112501 Copy
Species: Synthetic
Genetic Insert: Pveg-eGFP
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3185; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/06/185108 for bioRxiv preprint.
Proper citation: RRID:Addgene_112792 Copy
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