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Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54391 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59947 Copy
Species: Oryza sativa
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:9620; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25809382
Proper citation: RRID:Addgene_61466 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61649 Copy
Species: Auquorea victoria
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61655 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61703 Copy
Vector Backbone Description: Backbone Size:11775; Vector Backbone:pEarleyGate-202; Vector Types:Plant Expression, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61705 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69771 Copy
Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pCAMBIA1300; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26644504
Comments: See genbank file for best annotation of features.
Proper citation: RRID:Addgene_71275 Copy
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_71534 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9000; Vector Backbone:pBI101; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21343429
Comments: Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612
Proper citation: RRID:Addgene_71745 Copy
Vector Backbone Description: Backbone Size:7450; Vector Backbone:pDEST1K18ms; Vector Types:sucrose counter-selection allelic exchange vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34032522
Proper citation: RRID:Addgene_72646 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM1886.2
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84103 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM1886.1
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84102 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM165/166
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84091 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM169a
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84094 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM168
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84093 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM169d
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84095 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM171
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84098 Copy
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